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Additional investigations into the retention mechanism of hydrophilic interaction liquid chromatography by linear solvation energy relationships
journal contribution
posted on 2023-05-18, 00:55 authored by Schuster, G, Lindner, WIn analogy to our previous publication, the hydrophilic interaction liquid chromatography mechanism was examined in terms of hydrogen bonding, coulombic interactions and phase ratio using linear solvation energy relationships. At first, 23 commercially available and in-house synthesized chromatographic supports are discussed in order to obtain system constants at pH 5.0 with ammonium acetate as buffer salt. Subsequently we compared these outcomes with our former results obtained at pH 3.0 with ammonium formate as buffer additive. Goodness of fit in terms of the adjusted multiple correlation coefficient was found to be reduced under the new conditions. No universal model which simultaneously comprised acidic, basic and neutral analytes could be performed. A significant enhancement of the HILIC systems hydrogen bond basicity was found when changing the pH and buffer counter ions. Even though packing materials showed similar selectivity profiles during the collection of the experimental retention data, different forces were found to account for the overall retention (e.g. Shiseido PC HILIC and Nucleodur HILIC). This indicates that HILIC type selectivity is rather based on a sum of additive or multiplicative phenomena.
History
Publication title
Journal of Chromatography AVolume
1301Pagination
98-110ISSN
0021-9673Department/School
School of Natural SciencesPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Copyright 2013 Elsevier B.V.Repository Status
- Restricted