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Additional investigations into the retention mechanism of hydrophilic interaction liquid chromatography by linear solvation energy relationships

Citation

Schuster, G and Lindner, W, Additional investigations into the retention mechanism of hydrophilic interaction liquid chromatography by linear solvation energy relationships, Journal of Chromatography A, 1301 pp. 98-110. ISSN 0021-9673 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2013.05.065

Abstract

In analogy to our previous publication, the hydrophilic interaction liquid chromatography mechanism was examined in terms of hydrogen bonding, coulombic interactions and phase ratio using linear solvation energy relationships. At first, 23 commercially available and in-house synthesized chromatographic supports are discussed in order to obtain system constants at pH 5.0 with ammonium acetate as buffer salt. Subsequently we compared these outcomes with our former results obtained at pH 3.0 with ammonium formate as buffer additive. Goodness of fit in terms of the adjusted multiple correlation coefficient was found to be reduced under the new conditions. No universal model which simultaneously comprised acidic, basic and neutral analytes could be performed. A significant enhancement of the HILIC systems hydrogen bond basicity was found when changing the pH and buffer counter ions. Even though packing materials showed similar selectivity profiles during the collection of the experimental retention data, different forces were found to account for the overall retention (e.g. Shiseido PC HILIC and Nucleodur HILIC). This indicates that HILIC type selectivity is rather based on a sum of additive or multiplicative phenomena.

Item Details

Item Type:Refereed Article
Keywords:hydrophilic interaction liquid chromatography (HILIC), linear solvation energy relationship (LSER), solvation parameter model column characterization
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Schuster, G (Dr Georg Schuster)
ID Code:91509
Year Published:2013
Web of Science® Times Cited:18
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-05-21
Last Modified:2015-02-07
Downloads:0

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