eCite Digital Repository

An automated pH-controlled culture system for laboratory-based ocean acidification experiments


McGraw, C and Cornwall, CE and Reid, M and Currie, K and Hepburn, C and Boyd, PW and Hurd, CL and Hunter, K, An automated pH-controlled culture system for laboratory-based ocean acidification experiments, Limnology and Oceanography, 8, (December) pp. 686-694. ISSN 0024-3590 (2010) [Refereed Article]

Restricted - Request a copy

Copyright Statement

Copyright 2010 by the American Society of Limnology and Oceanography Inc.

Official URL:

DOI: doi:10.4319/lom.2010.8.686


Using a spectrophotometric pH measurement system, an automated 12-tank culture system was developed, which is capable of maintaining pH levels between 7.51 and 8.00 (local, in-situ pH) within 0.02 pH units at 15.1°C. The precise control of pH in the individual culture tanks was achieved through the addition of CO2- enriched seawater to unamended seawater. A feed-back system, which automatically adjusted the mixing ratio of unamended and CO2-enriched seawater, ensured that the pH in each tank was kept within 0.02 pH units of target values. After each tank adjustment, the spectrophotometric pH measurement was combined with a dissolved inorganic carbon measurement to automatically re-calculate carbonate chemistry in each tank. The system was used to study the growth of the geniculate coralline alga Arthrocardia corymbosa at pH levels of 7.52, 7.76, and 7.98.

Item Details

Item Type:Refereed Article
Keywords:Ocean acidification, pH, culture system, method development
Research Division:Environmental Sciences
Research Group:Climate change impacts and adaptation
Research Field:Ecological impacts of climate change and ecological adaptation
Objective Division:Environmental Policy, Climate Change and Natural Hazards
Objective Group:Adaptation to climate change
Objective Field:Ecosystem adaptation to climate change
UTAS Author:Cornwall, CE (Dr Chris Cornwall)
UTAS Author:Boyd, PW (Professor Philip Boyd)
UTAS Author:Hurd, CL (Professor Catriona Hurd)
ID Code:91477
Year Published:2010
Web of Science® Times Cited:52
Deposited By:IMAS Research and Education Centre
Deposited On:2014-05-20
Last Modified:2014-08-27

Repository Staff Only: item control page