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An automated pH-controlled culture system for laboratory-based ocean acidification experiments
Citation
McGraw, C and Cornwall, CE and Reid, M and Currie, K and Hepburn, C and Boyd, PW and Hurd, CL and Hunter, K, An automated pH-controlled culture system for laboratory-based ocean acidification experiments, Limnology and Oceanography, 8, (December) pp. 686-694. ISSN 0024-3590 (2010) [Refereed Article]
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Copyright Statement
Copyright 2010 by the American Society of Limnology and Oceanography Inc.
Official URL: http://www.aslo.org/
DOI: doi:10.4319/lom.2010.8.686
Abstract
Using a spectrophotometric pH measurement system, an automated 12-tank culture system was developed,
which is capable of maintaining pH levels between 7.51 and 8.00 (local, in-situ pH) within 0.02 pH units at
15.1°C. The precise control of pH in the individual culture tanks was achieved through the addition of CO2-
enriched seawater to unamended seawater. A feed-back system, which automatically adjusted the mixing ratio
of unamended and CO2-enriched seawater, ensured that the pH in each tank was kept within 0.02 pH units of
target values. After each tank adjustment, the spectrophotometric pH measurement was combined with a dissolved
inorganic carbon measurement to automatically re-calculate carbonate chemistry in each tank. The system
was used to study the growth of the geniculate coralline alga Arthrocardia corymbosa at pH levels of 7.52,
7.76, and 7.98.
Item Details
Item Type: | Refereed Article |
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Keywords: | Ocean acidification, pH, culture system, method development |
Research Division: | Environmental Sciences |
Research Group: | Climate change impacts and adaptation |
Research Field: | Ecological impacts of climate change and ecological adaptation |
Objective Division: | Environmental Policy, Climate Change and Natural Hazards |
Objective Group: | Adaptation to climate change |
Objective Field: | Ecosystem adaptation to climate change |
UTAS Author: | Cornwall, CE (Dr Chris Cornwall) |
UTAS Author: | Boyd, PW (Professor Philip Boyd) |
UTAS Author: | Hurd, CL (Professor Catriona Hurd) |
ID Code: | 91477 |
Year Published: | 2010 |
Web of Science® Times Cited: | 52 |
Deposited By: | IMAS Research and Education Centre |
Deposited On: | 2014-05-20 |
Last Modified: | 2014-08-27 |
Downloads: | 0 |
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