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An automated pH-controlled culture system for laboratory-based ocean acidification experiments

journal contribution
posted on 2023-05-18, 00:51 authored by McGraw, C, Cornwall, CE, Reid, M, Currie, K, Hepburn, C, Philip BoydPhilip Boyd, Catriona HurdCatriona Hurd, Hunter, K
Using a spectrophotometric pH measurement system, an automated 12-tank culture system was developed, which is capable of maintaining pH levels between 7.51 and 8.00 (local, in-situ pH) within 0.02 pH units at 15.1°C. The precise control of pH in the individual culture tanks was achieved through the addition of CO2- enriched seawater to unamended seawater. A feed-back system, which automatically adjusted the mixing ratio of unamended and CO2-enriched seawater, ensured that the pH in each tank was kept within 0.02 pH units of target values. After each tank adjustment, the spectrophotometric pH measurement was combined with a dissolved inorganic carbon measurement to automatically re-calculate carbonate chemistry in each tank. The system was used to study the growth of the geniculate coralline alga Arthrocardia corymbosa at pH levels of 7.52, 7.76, and 7.98.

History

Publication title

Limnology and Oceanography

Volume

8

Issue

December

Pagination

686-694

ISSN

0024-3590

Department/School

Institute for Marine and Antarctic Studies

Publisher

Amer Soc Limnology Oceanography

Place of publication

5400 Bosque Blvd, Ste 680, Waco, USA, Tx, 76710-4446

Rights statement

Copyright 2010 by the American Society of Limnology and Oceanography Inc.

Repository Status

  • Restricted

Socio-economic Objectives

Ecosystem adaptation to climate change

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