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Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures

Citation

Polinski, M and Bridle, A and Neumann, L and Nowak, B, Preliminary evidence of transcriptional immunomodulation by praziquantel in bluefin tuna and Atlantic salmon in vitro cultures, Fish and Shellfish Immunology, 38, (1) pp. 42-46. ISSN 1050-4648 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier Ltd.

DOI: doi:10.1016/j.fsi.2014.02.023

Abstract

Praziquantel (PZQ), long-used in veterinary and human medicine for the treatment of helminth parasites, is known to enhance humoral and cellular immune responsiveness in mammals but has unknown direct immunomodulatory capabilities in fish. In the present study, we examined the ability of PZQ to induce gene transcriptional changes in immune-competent primary tissue/organ cultures of two highly important yet evolutionarily discrete fish species Southern bluefin tuna Thunnus maccoyii and Atlantic salmon Salmo salar. These cultures consisted of mixed blood cell population for both species, as well as intestinal explants from bluefin. Although expression profiles varied between species and tissue/organ type, PZQ induced both T-cell receptor (more than twofold) and IL-8 transcriptional expression (more than fourfold). Additionally, increased expression of other inflammatory cytokines including IL-1β was detected in blood cell cultures from both species, and a general pattern of heightened antiviral signaling was observed. Specifically, elevated transcription of Type I (IFNα) and Type II (IFNγ) interferon in Atlantic salmon blood cultures along with elevated expression of MHC class I in blood cultures of both species. These findings provide preliminary evidence for direct immunomodulation by PZQ in fish and insight into its potential capacity as an immune stimulant/adjuvant in the rapidly expanding aquaculture industry.

Item Details

Item Type:Refereed Article
Keywords:Praziquantel, immunostimulant, gene expression, bluefin tuna, Atlantic salmon
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Tuna
Author:Polinski, M (Mr Mark Polinski)
Author:Bridle, A (Dr Andrew Bridle)
Author:Neumann, L (Mr Lukas Neumann)
Author:Nowak, B (Professor Barbara Nowak)
ID Code:91204
Year Published:2014
Web of Science® Times Cited:1
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-05-11
Last Modified:2017-11-04
Downloads:0

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