Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.

Item Type:	Refereed Article
Keywords:	AGD, Atlantic salmon, mRNA, gene expression, 2D quantitative RT-PCR, inflammatory response
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Fisheries sciences
Research Field:	Aquaculture
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Fisheries - aquaculture
Objective Field:	Aquaculture fin fish (excl. tuna)
UTAS Author:	Pennacchi, Y (Ms Ylenia Pennacchi)
UTAS Author:	Leef, MJ (Dr Melanie Leef)
UTAS Author:	Crosbie, PBB (Dr Philip Crosbie)
UTAS Author:	Nowak, BF (Professor Barbara Nowak)
UTAS Author:	Bridle, AR (Associate Professor Andrew Bridle)
ID Code:	91201
Year Published:	2014
Web of Science® Times Cited:	37
Deposited By:	NC Marine Conservation and Resource Sustainability
Deposited On:	2014-05-11
Last Modified:	2017-11-04
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