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Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L

Citation

Pennacchi, Y and Leef, MJ and Crosbie, PBB and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L, Fish and Shellfish Immunology, 36, (2) pp. 563-570. ISSN 1050-4648 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier

DOI: doi:10.1016/j.fsi.2013.12.013

Abstract

Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.

Item Details

Item Type:Refereed Article
Keywords:AGD, Atlantic salmon, mRNA, gene expression, 2D quantitative RT-PCR, inflammatory response
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Pennacchi, Y (Ms Ylenia Pennacchi)
Author:Leef, MJ (Dr Melanie Leef)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Bridle, AR (Dr Andrew Bridle)
ID Code:91201
Year Published:2014
Web of Science® Times Cited:11
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-05-11
Last Modified:2017-11-04
Downloads:0

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