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Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L
Citation
Pennacchi, Y and Leef, MJ and Crosbie, PBB and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L, Fish and Shellfish Immunology, 36, (2) pp. 563-570. ISSN 1050-4648 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 Elsevier
DOI: doi:10.1016/j.fsi.2013.12.013
Abstract
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.
Item Details
Item Type: | Refereed Article |
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Keywords: | AGD, Atlantic salmon, mRNA, gene expression, 2D quantitative RT-PCR, inflammatory response |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture fin fish (excl. tuna) |
UTAS Author: | Pennacchi, Y (Ms Ylenia Pennacchi) |
UTAS Author: | Leef, MJ (Dr Melanie Leef) |
UTAS Author: | Crosbie, PBB (Dr Philip Crosbie) |
UTAS Author: | Nowak, BF (Professor Barbara Nowak) |
UTAS Author: | Bridle, AR (Associate Professor Andrew Bridle) |
ID Code: | 91201 |
Year Published: | 2014 |
Web of Science® Times Cited: | 43 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2014-05-11 |
Last Modified: | 2017-11-04 |
Downloads: | 0 |
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