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Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L

journal contribution
posted on 2023-05-18, 00:32 authored by Pennacchi, Y, Melanie Leef, Crosbie, PBB, Barbara NowakBarbara Nowak, Andrew BridleAndrew Bridle
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.

History

Publication title

Fish and Shellfish Immunology

Volume

36

Pagination

563-570

ISSN

1050-4648

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier

Place of publication

24-28 Oval Rd, London, England, Nw1 7Dx

Rights statement

Copyright 2014 Elsevier

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna)

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