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Simultaneous determination of organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale by RP-HPLC after solid-phase extraction with nano-sized hydroxyapatite
journal contribution
posted on 2023-05-18, 00:25 authored by Wei, W, Wei, Z, Wang, Y, Baker, AJM, Zhao, H, Li, H, Hu, FAn analytical approach based on solid-phase extraction (SPE) with nanosized hydroxyapatite and reversed-phase high performance liquid chromatography (RP-HPLC) for the simultaneous determination of organic acids (oxalic, malic, malonic, citric, and fumaric) and nitrate in xylem saps of the hyperaccumulator Alyssum murale is presented in this study. The optimum experimental conditions for the separation and determination of organic acids and nitrate are studied. The interference from nitrate on oxalic acid in RP-HPLC is eliminated by SPE with nanosized hydroxyapatite, and the simultaneous determination of organic acids and nitrate is achieved by RP-HPLC at the optimum chromatographic conditions. The accuracy of the method is confirmed with an average recovery ranging between 95.2% and 99.8%, the relative standard deviations (RSD) are less than 2.0%. This method is successfully applied to determine the organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale.
History
Publication title
Journal of Chromatographic ScienceVolume
48Issue
10Pagination
840-847ISSN
0021-9665Department/School
School of Natural SciencesPublisher
Preston Publications IncPlace of publication
7800 Merrimac Ave Po Box 48312, Niles, USA, Il, 60648Rights statement
Copyright 2010 Oxford University PressRepository Status
- Restricted