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Simultaneous determination of organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale by RP-HPLC after solid-phase extraction with nano-sized hydroxyapatite

journal contribution
posted on 2023-05-18, 00:25 authored by Wei, W, Wei, Z, Wang, Y, Baker, AJM, Zhao, H, Li, H, Hu, F
An analytical approach based on solid-phase extraction (SPE) with nanosized hydroxyapatite and reversed-phase high performance liquid chromatography (RP-HPLC) for the simultaneous determination of organic acids (oxalic, malic, malonic, citric, and fumaric) and nitrate in xylem saps of the hyperaccumulator Alyssum murale is presented in this study. The optimum experimental conditions for the separation and determination of organic acids and nitrate are studied. The interference from nitrate on oxalic acid in RP-HPLC is eliminated by SPE with nanosized hydroxyapatite, and the simultaneous determination of organic acids and nitrate is achieved by RP-HPLC at the optimum chromatographic conditions. The accuracy of the method is confirmed with an average recovery ranging between 95.2% and 99.8%, the relative standard deviations (RSD) are less than 2.0%. This method is successfully applied to determine the organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale.

History

Publication title

Journal of Chromatographic Science

Volume

48

Issue

10

Pagination

840-847

ISSN

0021-9665

Department/School

School of Natural Sciences

Publisher

Preston Publications Inc

Place of publication

7800 Merrimac Ave Po Box 48312, Niles, USA, Il, 60648

Rights statement

Copyright 2010 Oxford University Press

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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