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Simultaneous determination of organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale by RP-HPLC after solid-phase extraction with nano-sized hydroxyapatite
Citation
Wei, W and Wei, Z and Wang, Y and Baker, AJM and Zhao, H and Li, H and Hu, F, Simultaneous determination of organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale by RP-HPLC after solid-phase extraction with nano-sized hydroxyapatite, Journal of Chromatographic Science, 48, (10) pp. 840-847. ISSN 0021-9665 (2010) [Refereed Article]
Copyright Statement
Copyright 2010 Oxford University Press
DOI: doi:10.1093/chromsci/48.10.840
Abstract
An analytical approach based on solid-phase extraction (SPE) with nanosized hydroxyapatite and reversed-phase high performance liquid chromatography (RP-HPLC) for the simultaneous determination of organic acids (oxalic, malic, malonic, citric, and fumaric) and nitrate in xylem saps of the hyperaccumulator Alyssum murale is presented in this study. The optimum experimental conditions for the separation and determination of organic acids and nitrate are studied. The interference from nitrate on oxalic acid in RP-HPLC is eliminated by SPE with nanosized hydroxyapatite, and the simultaneous determination of organic acids and nitrate is achieved by RP-HPLC at the optimum chromatographic conditions. The accuracy of the method is confirmed with an average recovery ranging between 95.2% and 99.8%, the relative standard deviations (RSD) are less than 2.0%. This method is successfully applied to determine the organic acids and nitrate in xylem saps of the hyperaccumulator Alyssum murale.
Item Details
Item Type: | Refereed Article |
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Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Wei, Z (Professor Zhenggui Wei) |
ID Code: | 91138 |
Year Published: | 2010 |
Web of Science® Times Cited: | 6 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2014-05-09 |
Last Modified: | 2014-06-16 |
Downloads: | 0 |
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