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Rapid inexpensive genome-wide association using pooled whole blood
Citation
Craig, JE and Hewitt, AW and McMellon, AE and Henders, AK and Ma, L and Wallace, L and Sharma, S and Burdon, KP and Visscher, PM and Montgomery, GW and MacGregor, S, Rapid inexpensive genome-wide association using pooled whole blood, Genome Research, 19, (11) pp. 2075-2080. ISSN 1088-9051 (2009) [Refereed Article]
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Copyright Statement
Copyright 2009 Cold Spring Harbor Laboratory Press
DOI: doi:10.1101/gr.094680.109
Abstract
Genome-wide association studies (GWAS) have now successfully identified important genetic variants associated with many human traits and diseases. The high cost of genotyping arrays in large data sets remains the major barrier to wider utilization of GWAS. We have developed a novel method in which whole blood from cases and controls, respectively, is pooled prior to DNA extraction for genotyping. We demonstrate proof of principle by clearly identifying the associated variants for eye color, age-related macular degeneration, and pseudoexfoliation syndrome in cohorts not previously studied. Blood pooling has the potential to reduce GWAS cost by several orders of magnitude and dramatically shorten gene discovery time. This method has profound implications for translation of modern genetic approaches to a multitude of diseases and traits yet to be analyzed by GWAS, and will enable developing nations to participate in GWAS.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Ophthalmology and optometry |
Research Field: | Ophthalmology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Hewitt, AW (Professor Alex Hewitt) |
UTAS Author: | Burdon, KP (Professor Kathryn Burdon) |
ID Code: | 91097 |
Year Published: | 2009 |
Web of Science® Times Cited: | 33 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2014-05-07 |
Last Modified: | 2014-10-06 |
Downloads: | 0 |
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