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Macroporous monolithic supports for affinity chromatography
journal contribution
posted on 2023-05-18, 00:18 authored by Arrua, RD, Alvarez Igarzabal, CIIn the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.
History
Publication title
Journal of Separation ScienceVolume
34Issue
16-17Pagination
1974-1987ISSN
1615-9306Department/School
School of Natural SciencesPublisher
GermanyPlace of publication
Wiley - VCH Verlag GmbH & CoRights statement
Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted