eCite Digital Repository
Macroporous monolithic supports for affinity chromatography
Citation
Arrua, RD and Alvarez Igarzabal, CI, Macroporous monolithic supports for affinity chromatography, Journal of Separation Science, 34, (16-17) pp. 1974-1987. ISSN 1615-9306 (2011) [Refereed Article]
Copyright Statement
Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: doi:10.1002/jssc.201100197
Abstract
In the early 1990s, three research groups simultaneously developed continuous macroporous
rod-shaped polymeric systems to eliminate the problem of flow through the
interparticle spaces generally presented by the chromatography columns that use particles
as filler. The great advantage of those materials, forming a continuous phase rod, is to
increase the mass transfer by convective transport, as the mobile phase is forced to go
through all means of separation, in contrast to particulate media where the mobile phase
flows through the interparticle spaces. Due to their special characteristics, the monolithic
polymers are used as base-supports in different separation techniques, those chromatographic
processes being the most important and, to a greater extent, those involving the
separation of biomolecules as in the case of affinity chromatography. This mini-review
reports the contributions of several groups to the development of macroporous monoliths
and their modification by immobilization of specific ligands on the products for their
application in affinity chromatography.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | affinity chromatography, macroporous materials, monolithic supports |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Arrua, RD (Dr Dario Arrua) |
ID Code: | 91071 |
Year Published: | 2011 |
Web of Science® Times Cited: | 32 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2014-05-07 |
Last Modified: | 2014-06-10 |
Downloads: | 0 |
Repository Staff Only: item control page