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Stability of the combination of ceftazidime and cephazolin in icodextrin or pH neutral peritoneal dialysis solution

Citation

Patel, RP and Shastri, MD and Bakkari, M and Wanandy, T and Jose, MD, Stability of the combination of ceftazidime and cephazolin in icodextrin or pH neutral peritoneal dialysis solution, Peritoneal Dialysis International, 34, (2) pp. 212-218. ISSN 0896-8608 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 International Society for Peritoneal Dialysis

DOI: doi:10.3747/pdi.2013.00034

Abstract

Introduction: The objective of this study was to investigate the stability of ceftazidime and cephazolin in a 7.5% icodextrin or pH neutral peritoneal dialysis (PD) solution.

Methods: Ceftazidime and cephazolin were injected into either a 7.5% icodextrin or pH neutral PD bag to obtain the concentration of 125 mg/L of each antibiotic. A total of nine 7.5% icodextrin or pH neutral PD bags containing ceftazidime and cephazolin were prepared and stored at 1 of 3 different temperatures: 4C in a domestic refrigerator; 25C at room temperature; or 37C (body temperature) in an incubator. An aliquot was withdrawn immediately before (0 hour) or after 12, 24, 48, 96, 120, 144, 168 and 336 hours of storage. Each sample was analyzed in duplicate for the concentration of ceftazidime and cephazolin using a stability-indicating high-performance liquid chromatography technique. Ceftazidime and cephazolin were considered stable if they retained more than 90% of their initial concentration. Samples were also assessed for pH, colour changes and evidence of precipitation immediately after preparation and on each day of analysis.

Results: Ceftazidime and cephazolin in both types of PD solution retained more than 90% of their initial concentration for 168 and 336 hours respectively when stored at 4C. Both of the antibiotics lost more than 10% of the initial concentration after 24 hours of storage at 25 or 37C. There was no evidence of precipitation at any time under the tested storage conditions. Change in the pH and color was observed at 25 and 37C, but not at 4C.

Conclusion: Premixed ceftazidime and cephazolin in a 7.5% icodextrin or pH neutral PD solution is stable for at least 168 hours when refrigerated. This allows the preparation of PD bags in advance, avoiding the necessity for daily preparation. Both the antibiotics are stable for at least 24 hours at 25 and 37C, permitting storage at room temperature and pre-warming of PD bags to body temperature prior to its administration.

Item Details

Item Type:Refereed Article
Keywords:Ceftazidime, cephazolin, icodextrin, pH neutral, peritoneal dialysis, stability, high-performance liquid chromatography
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Nephrology and Urology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Urogenital System and Disorders
UTAS Author:Patel, RP (Dr Rahul Patel)
UTAS Author:Shastri, MD (Mr Madhur Shastri)
UTAS Author:Bakkari, M (Mr Mohammed Bakkari)
UTAS Author:Wanandy, T (Mr Troy Wanandy)
UTAS Author:Jose, MD (Professor Matthew Jose)
ID Code:90562
Year Published:2014
Web of Science® Times Cited:6
Deposited By:Medicine
Deposited On:2014-04-10
Last Modified:2017-11-02
Downloads:0

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