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The iterative regression design developed earlier for high performance liquid chromatography (HPLC) has been applied successfully to the optimization of the separation of 11 metal ions by capillary electrophoresis. The parameters used for optimization were pH (over the range 3.5-5.0) and the concentration of 2-hydroxyisobutyric acid (HIBA) (2-20 mM) used as the complex-forming ligand added to the background electrolyte. Five initial experiments were performed and the response surface was calculated using the relative resolution product as criterion. Three additional experiments were sufficient to establish a stable optimum, with which all 11 metal ions were separated and well spread in the electropherogram. The total analysis time was less than 5 min.

Item Type:	Refereed Article
Keywords:	Capillary electrophoresis • Metal ions • Iterative regression optimization • 2-Hydroxyisobutyric acid • Metal ligand complexes
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Instrumental Methods (excl. Immunological and Bioassay Methods)
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Andersson, PE (Dr Andersson)
Author:	Haddad, PR (Professor Paul Haddad)
ID Code:	9009
Year Published:	1996
Web of Science® Times Cited:	9
Deposited By:	Chemistry
Deposited On:	1996-08-01
Last Modified:	2010-03-10
Downloads:	0