eCite Digital Repository

Enantiospecific gas chromatographic mass-spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma - application to protein binding studies

Citation

Jack, DS and Rumble, RH and Davies, NW and Francis, HW, Enantiospecific gas chromatographic mass-spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma - application to protein binding studies, Journal of Chromatography A, 584 pp. 189-197. ISSN 0021-9673 (1992) [Refereed Article]

Abstract

A method for the enantiospecific qunatitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected ion monitoring. The assay i scapabl eof quantifying ketoprefen (2 ng/mL) and iboprofen (3ng/mL) enantiomers from a 200-uL sample of synovial fluid or plasma and is particularly suitable for protein binding studies.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Pharmaceutical sciences
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Jack, DS (Mr David Jack)
UTAS Author:Rumble, RH (Dr Roger Rumble)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
UTAS Author:Francis, HW (Dr Hilton Francis)
ID Code:90014
Year Published:1992
Deposited By:Central Science Laboratory
Deposited On:2014-03-24
Last Modified:2014-03-24
Downloads:0

Repository Staff Only: item control page