eCite Digital Repository

Respirometry: Correcting for diffusion and validating the use of plastic multiwell plates with integrated optodes

Citation

Polymeropoulos, ET and Elliott, NG and Wotherspoon, SJ and Frappell, PB, Respirometry: Correcting for diffusion and validating the use of plastic multiwell plates with integrated optodes, Physiological and Biochemical Zoology, 86, (5) pp. 588-592. ISSN 1522-2152 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 University of Chicago

DOI: doi:10.1086/671800

Abstract

Polystyrene multiwell plates with integrated optodes act as multiple closed-system respirometers that enable the simultaneous measurement of oxygen consumption in small animals. However, the diffusion of oxygen through polystyrene needs to be taken into consideration. Here we provide an equation that accounts for the empirically determined rate of oxygen through a polystyrene well when calculating the instantaneous rate of oxygen consumption. Furthermore, we describe a novel method of calibrating a small respirometer for accuracy using micro-osmotic pumps containing an oxygen scavenger, which is delivered at a constant rate and therefore yields a constant rate of oxygen consumption in an airtight system.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Animal Physiology - Systems
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Physical Sciences
Author:Polymeropoulos, ET (Dr Elias Polymeropoulos)
Author:Wotherspoon, SJ (Dr Simon Wotherspoon)
Author:Frappell, PB (Professor Peter Frappell)
ID Code:89887
Year Published:2013
Web of Science® Times Cited:3
Deposited By:Zoology
Deposited On:2014-03-18
Last Modified:2017-11-01
Downloads:0

Repository Staff Only: item control page