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Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides
Citation
Ordonez, NM and Shabala, L and Gehring, C and Shabala, S, Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides, Cyclic Nucleotide Signaling in Plants: Methods and Protocols, Springer, C Gehring (ed), Heidelberg, Germany, pp. 95-106. ISBN 978-1-62703-440-1 (2013) [Research Book Chapter]
Copyright Statement
Copyright 2013 Springer
DOI: doi:10.1007/978-1-62703-441-8_7
Abstract
Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular. © Springer Science+Business Media New York 2013.
Item Details
Item Type: | Research Book Chapter |
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Keywords: | membrane transport, MIFE, cell signaling, cyclic nucleotides, microelectrodes, ion fluxes, stress responses, channels, membrane transporters |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Environmentally sustainable plant production |
Objective Field: | Environmentally sustainable plant production not elsewhere classified |
UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
UTAS Author: | Shabala, S (Professor Sergey Shabala) |
ID Code: | 89647 |
Year Published: | 2013 |
Deposited By: | Tasmanian Institute of Agriculture |
Deposited On: | 2014-03-11 |
Last Modified: | 2014-08-07 |
Downloads: | 0 |
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