Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular. © Springer Science+Business Media New York 2013.

Item Type:	Research Book Chapter
Keywords:	membrane transport, MIFE, cell signaling, cyclic nucleotides, microelectrodes, ion fluxes, stress responses, channels, membrane transporters
Research Division:	Biological Sciences
Research Group:	Plant biology
Research Field:	Plant physiology
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Environmentally sustainable plant production
Objective Field:	Environmentally sustainable plant production not elsewhere classified
UTAS Author:	Shabala, L (Associate Professor Lana Shabala)
UTAS Author:	Shabala, S (Professor Sergey Shabala)
ID Code:	89647
Year Published:	2013
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2014-03-11
Last Modified:	2014-08-07
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