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A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef
Citation
Torda, G and Schmidt-Roach, S and Peplow, LM and Lundgren, P and van Oppen, MJH, A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef, PLoS ONE, 8, (3) Article e58447. ISSN 1932-6203 (2013) [Refereed Article]
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Copyright Statement
Copyright 2013 Torda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: doi:10.1371/journal.pone.0058447
Abstract
Pocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Genetics |
| Research Field: | Genetics not elsewhere classified |
| Objective Division: | Environmental Management |
| Objective Group: | Coastal and estuarine systems and management |
| Objective Field: | Coastal or estuarine biodiversity |
| UTAS Author: | Schmidt-Roach, S (Mr Sebastian Schmidt-Roach) |
| ID Code: | 89592 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 20 |
| Deposited By: | IMAS Research and Education Centre |
| Deposited On: | 2014-03-07 |
| Last Modified: | 2014-12-10 |
| Downloads: | 266 View Download Statistics |
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