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A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef

Citation

Torda, G and Schmidt-Roach, S and Peplow, LM and Lundgren, P and van Oppen, MJH, A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef, PLoS ONE, 8, (3) Article e58447. ISSN 1932-6203 (2013) [Refereed Article]


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Copyright 2013 Torda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

DOI: doi:10.1371/journal.pone.0058447

Abstract

Pocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Coastal and Estuarine Flora, Fauna and Biodiversity
UTAS Author:Schmidt-Roach, S (Mr Sebastian Schmidt-Roach)
ID Code:89592
Year Published:2013
Web of Science® Times Cited:16
Deposited By:IMAS Research and Education Centre
Deposited On:2014-03-07
Last Modified:2014-12-10
Downloads:238 View Download Statistics

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