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On-line sequential injection-capillary electrophoresis for near-real-time monitoring of extracellular lactate in cell culture flasks
Citation
Alhusban, AA and Gaudry, A and Breadmore, MC and Gueven, N and Guijt, RM, On-line sequential injection-capillary electrophoresis for near-real-time monitoring of extracellular lactate in cell culture flasks, Journal of Chromatography A, 1323 pp. 157-162. ISSN 0021-9673 (2014) [Refereed Article]
Copyright Statement
Copyright 2013 Elsevier
DOI: doi:10.1016/j.chroma.2013.11.006
Abstract
Cell culture has replaced many in vivo studies because of ethical and regulatory measures as well as the possibility of increased throughput. Analytical assays to determine (bio)chemical changes are often based on end-point measurements rather than on a series of sequential determinations. The purpose of this work is to develop an analytical system for monitoring cell culture based on sequential injection-capillary electrophoresis (SI-CE) with capacitively coupled contactless conductivity detection (C4D). The system was applied for monitoring lactate production, an important metabolic indicator, during mammalian cell culture. Using a background electrolyte consisting of 25 mM tris(hydroxymethyl)aminomethane, 35 mM cyclohexyl-2-aminoethanesulfonic acid with 0.02% poly(ethyleneimine) (PEI) at pH 8.65 and a multilayer polymer coated capillary, lactate could be resolved from other compounds present in media with relative standard deviations 0.07% for intraday electrophoretic mobility and an analysis time of less than 10 min. Using the human embryonic kidney cell line HEK293, lactate concentrations in the cell culture medium were measured every 20 min over 3 days, requiring only 8.73 μL of sample per run. Combining simplicity, portability, automation, high sample throughput, low limits of detection, low sample consumption and the ability to up- and outscale, this new methodology represents a promising technique for near real-time monitoring of chemical changes in diverse cell culture applications.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | real time monitoring, sequential injection capillary electrophoresis, online monitoring, bio process monitoring, capillary electrophoresis, cell culture |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Separation science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the chemical sciences |
| UTAS Author: | Alhusban, AA (Mr Ala Alhusban) |
| UTAS Author: | Gaudry, A (Mr Adam Gaudry) |
| UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
| UTAS Author: | Gueven, N (Dr Nuri Guven) |
| UTAS Author: | Guijt, RM (Dr Rosanne Guijt) |
| ID Code: | 89564 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 24 |
| Deposited By: | Pharmacy |
| Deposited On: | 2014-03-07 |
| Last Modified: | 2017-11-06 |
| Downloads: | 3 View Download Statistics |
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