eCite Digital Repository

Downregulation of IgE antibody and allergic responses in the lung by epidermal biolistic microparticle delivery


Kendall, M and Mitchell, TJ and Costigan, G and Armitage, M and Lenzo, JC and Thomas, JA and von Garnier, C and Zosky, GR and Turner, DJ and Stumbles, PA and Sly, PD and Holt, PG and Thomas, WR, Downregulation of IgE antibody and allergic responses in the lung by epidermal biolistic microparticle delivery, The Journal of Allergy and Clinical Immunology, 117, (2) pp. 275-82. ISSN 0091-6749 (2006) [Refereed Article]

DOI: doi:10.1016/j.jaci.2005.09.049


BACKGROUND: Biolistic injections provide a needle-free delivery of antigen-laden microparticles to the epithelium. The precision of the injection preferentially targets the Langerhans cell network, which, although ideal for vaccination, might not be suitable for the downregulation of immune responses in immunotherapy. OBJECTIVE: We sought to determine the ability of biolistic injection of antigen into the epithelium of sensitized mice to inhibit IgE antibody and lung inflammatory responses produced by further exposure to antigen. METHODS: Mice were sensitized by means of a needle injection of ovalbumin (OVA) in alum and given a series of biolistic injections of OVA or vehicle control, followed by a boost of OVA in alum. Serum IgE and IgG antibodies were measured before and after the boost. The mice were then challenged intranasally, and the infiltration of inflammatory cells was measured by means of bronchoalveolar lavage. Airway reactivity of the challenged mice was measured by examining responses to methacholine with forced oscillatory techniques. RESULTS: Biolistic injection of OVA into the dorsal skin of sensitized mice markedly inhibited IgE and IgG1 antibody responses induced by boosting. IgG2a antibody responses were reduced rather than stimulated. The eosinophilic inflammation in the bronchoalveolar lavage fluid induced by intranasal challenge was also markedly inhibited. Lung hyperreactivity showed an initial increase and then a decrease of responsiveness to methacholine, with elastance returning to the level of unsensitized mice. Biolistic injection into the buccal epithelium was also inhibitory. CONCLUSIONS: Biolistic injection of allergen inhibited the boosting of IgE antibody and eosinophilic lung inflammatory responses without inducing T(H)1 immunity.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:89503
Year Published:2006
Web of Science® Times Cited:11
Deposited By:Medicine
Deposited On:2014-03-06
Last Modified:2014-03-06

Repository Staff Only: item control page