eCite Digital Repository

Protective mechanical ventilation does not exacerbate lung function impairment or lung inflammation following influenza A infection


Zosky, GR and Cannizzaro, V and Hantos, Z and Sly, PD, Protective mechanical ventilation does not exacerbate lung function impairment or lung inflammation following influenza A infection, Journal of Applied Physiology, 107, (5) pp. 1472-8. ISSN 8750-7587 (2009) [Refereed Article]

DOI: doi:10.1152/japplphysiol.00393.2009.


The degree to which mechanical ventilation induces ventilator-associated lung injury is dependent on the initial acute lung injury (ALI). Viral-induced ALI is poorly studied, and this study aimed to determine whether ALI induced by a clinically relevant infection is exacerbated by protective mechanical ventilation. Adult female BALB/c mice were inoculated with 10(4.5) plaque-forming units of influenza A/Mem/1/71 in 50 microl of medium or medium alone. This study used a protective ventilation strategy, whereby mice were anesthetized, tracheostomized, and mechanically ventilated for 2 h. Lung mechanics were measured periodically throughout the ventilation period using a modification of the forced oscillation technique to obtain measures of airway resistance and coefficients of tissue damping and tissue elastance. Thoracic gas volume was measured and used to obtain specific airway resistance, tissue damping, and tissue elastance. At the end of the ventilation period, a bronchoalveolar lavage sample was collected to measure inflammatory cells, macrophage inflammatory protein-2, IL-6, TNF-alpha, and protein leak. Influenza infection caused significant increases in inflammatory cells, protein leak, and deterioration in lung mechanics that were not exacerbated by mechanical ventilation, in contrast to previous studies using bacterial and mouse-specific viral infection. This study highlighted the importance of type and severity of lung injury in determining outcome following mechanical ventilation.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:89474
Year Published:2009
Web of Science® Times Cited:15
Deposited By:Medicine
Deposited On:2014-03-05
Last Modified:2014-03-05

Repository Staff Only: item control page