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Mechanism of formation of system peaks in ion-exclusion chromatography
Citation
Novic, M and Haddad, PR, Mechanism of formation of system peaks in ion-exclusion chromatography, Journal of Chromatography A, 1305 pp. 188-192. ISSN 0021-9673 (2013) [Refereed Article]
Copyright Statement
Copyright 2013 Elsevier
DOI: doi:10.1016/j.chroma.2013.07.041
Abstract
Single or multiple system peaks can be observed in ion-exclusion chromatography (IEC) based on whether
the eluent is composed of single or multiple active eluent constituents. It was confirmed experimentally
that the number of system peaks is always equal to or lower than (when co-elution occurs) the number
of active eluent components. Positive and negative system peaks can be recorded in the IEC systems.
Negative system peaks can be expected for each eluent component that is also present in the injected
sample plug at a lower concentration than in the eluent. In the opposite case, where the eluent components
in the injected sample plug are present at a higher concentration than in the eluent, positive
system peaks will be recorded. The retention times of individual system peaks are not dependent on
each other, but rather depend on the capacity of the column toward the individual eluent component.
This capacity depends on the nature of the eluent component and can be concentration-dependent (e.g.
for 2,6-pyridinedicarboxylic acid) or concentration-independent (e.g. for acetic acid). The higher the
column capacity, the longer the retention time of the corresponding system peak produced by that eluent
component. The positive and negative system peaks are the result of column re-equilibration to an
injected sample containing a higher or lower concentration of the eluent component, respectively. In
general, positive system peaks have longer retention times than negative system peaks. The larger the
concentration-dependent capacity of the IEC stationary phase toward an individual eluent component,
the larger the retention time difference between positive and negative system peaks.
Item Details
Item Type: | Refereed Article |
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Keywords: | system peaks, formation mechanism, ion exclusion chromatography, vacancy ion exclusion chromatography |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Haddad, PR (Professor Paul Haddad) |
ID Code: | 89401 |
Year Published: | 2013 |
Web of Science® Times Cited: | 1 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2014-03-04 |
Last Modified: | 2014-05-27 |
Downloads: | 0 |
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