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Effects of eluent temperature and elution bandwidth on detection response for aerosol-based detectors


Khandagale, MM and Hutchinson, JP and Dicinoski, GW and Haddad, PR, Effects of eluent temperature and elution bandwidth on detection response for aerosol-based detectors, Journal of Chromatography A, 1308 pp. 96-103. ISSN 0021-9673 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier

DOI: doi:10.1016/j.chroma.2013.07.111


Aerosol detectors provide generally uniform response for most analytes, independent of their optical properties, and have the advantage of being compatible with elevated temperature mobile phases. Therefore, aerosol detectors present an attractive detection alternative for high temperature liquid chromatography (HTLC) separations. The present study has investigated the effects of HTLC conditions using aqueous mobile phases on the detection response of an evaporative light-scattering detector (ELSD) and a corona-charged aerosol detector (C-CAD). The response of the ELSD was increased up to 5-fold by increasing the separation temperature from 30C to 180C. The C-CAD showed much smaller increases in response under the same conditions. This increase in response was found not to result from the increased temperature for the mobile phase but rather from compression of the elution bandwidth at elevated temperature. The effect of bandwidth on detector response was confirmed using flow-injection studies in which the same amount of analyte was introduced into the detector at varying bandwidths. Furthermore, it is shown that a temperature gradient can be used to counteract the effects of varying bandwidths associated with isocratic-isothermal separations, with relatively constant bandwidth and detector response being observed with appropriate temperature gradients. This study demonstrates the necessity to consider the elution bandwidth in HTLC-aerosol detector analysis.

Item Details

Item Type:Refereed Article
Keywords:elution bandwidth, HTLC, ELSD, C-CAD, temperature gradient separation, band dispersion
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Khandagale, MM (Mr Manish Khandagale)
UTAS Author:Hutchinson, JP (Dr Joseph Hutchinson)
UTAS Author:Dicinoski, GW (Associate Professor Gregory Dicinoski)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:89392
Year Published:2013
Funding Support:Australian Research Council (LP0884030)
Web of Science® Times Cited:7
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-03-04
Last Modified:2014-05-27

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