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Potential of Capillary Electrophoresis (CE) and Chip-CE with Dual Detection (Capacitively-Coupled Contactless Conductivity Detection (C4D) and Fluorescence Detection) for Monitoring of Nicotine and Cotinine Derivatization

Citation

Nuchtavorn, N and Ryvolova, M and Bek, F and Macka, M and Phechkrajang, C and Suntornsuk, L, Potential of Capillary Electrophoresis (CE) and Chip-CE with Dual Detection (Capacitively-Coupled Contactless Conductivity Detection (C4D) and Fluorescence Detection) for Monitoring of Nicotine and Cotinine Derivatization, Analytical Sciences, 29, (3) pp. 339-344. ISSN 0910-6340 (2013) [Refereed Article]


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Copyright Statement

Copyright 2013 The Japan Society for Analytical Chemistry

DOI: doi:10.2116/analsci.29.339

Abstract

Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C4D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C4D in 7 min (Rs > 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD < 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (< 7.5 μM) and precision (%RSD < 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization. © The Japan Society for Analytical Chemistry.

Item Details

Item Type:Refereed Article
Keywords:capillary electrophoresis (CE), chip-CE, capacitively-coupled contactless conductivity detection (C4D), fluorescence detection, derivatization, nicotine, cotinine
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Macka, M (Professor Mirek Macka)
ID Code:89297
Year Published:2013
Funding Support:Australian Research Council (FT120100559)
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-02-28
Last Modified:2014-06-06
Downloads:225 View Download Statistics

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