Preview

PDF 1Mb

Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C4D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C⁴D in 7 min (R_s > 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD < 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (< 7.5 μM) and precision (%RSD < 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization. © The Japan Society for Analytical Chemistry.

Item Type:	Refereed Article
Keywords:	capillary electrophoresis (CE), chip-CE, capacitively-coupled contactless conductivity detection (C4D), fluorescence detection, derivatization, nicotine, cotinine
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Macka, M (Professor Mirek Macka)
ID Code:	89297
Year Published:	2013
Funding Support:	Australian Research Council (FT120100559)
Web of Science® Times Cited:	13
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2014-02-28
Last Modified:	2014-06-06
Downloads:	369 View Download Statistics