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Ion flux measurements using the MIFE technique
chapter
posted on 2023-05-22, 21:27 authored by Sergey ShabalaSergey Shabala, Svetlana ShabalaSvetlana Shabala, Bose, J, Tracey Cuin, Ian NewmanIan NewmanNoninvasive microelectrode ion flux measurements (the MIFE™ technique) allow the concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. The MIFE technique has become a popular tool for studying the adaptive responses of plant cells and tissues to a large number of abiotic and biotic stresses. This chapter briefly summarizes some key findings on spatial and temporal organization of plant nutrient acquisition obtained by the MIFE technique, as well as the MIFE contribution towards elucidating the mechanisms behind a plant's perception and signaling of major abiotic stresses. The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for mapping root ion flux profiles and studying transient ion flux kinetics are given.
Funding
Australian Academy of Science
History
Publication title
Plant Mineral Nutrients: Methods and ProtocolsVolume
953Editors
FJM MaathuisPagination
171-183ISBN
978-1-62703-151-6Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Humana PressPlace of publication
United States of AmericaExtent
18Rights statement
Copyright 2013 Springer Science+ Business Media, LLC (Information transferred from record 84137, which was the same paper but which was originally entered as an A1, and which has been deleted. HW 28/2/14)Repository Status
- Restricted