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Ion flux measurements using the MIFE technique

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posted on 2023-05-22, 21:27 authored by Sergey ShabalaSergey Shabala, Svetlana ShabalaSvetlana Shabala, Bose, J, Tracey Cuin, Ian NewmanIan Newman
Noninvasive microelectrode ion flux measurements (the MIFE™ technique) allow the concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. The MIFE technique has become a popular tool for studying the adaptive responses of plant cells and tissues to a large number of abiotic and biotic stresses. This chapter briefly summarizes some key findings on spatial and temporal organization of plant nutrient acquisition obtained by the MIFE technique, as well as the MIFE contribution towards elucidating the mechanisms behind a plant's perception and signaling of major abiotic stresses. The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for mapping root ion flux profiles and studying transient ion flux kinetics are given.

Funding

Australian Academy of Science

History

Publication title

Plant Mineral Nutrients: Methods and Protocols

Volume

953

Editors

FJM Maathuis

Pagination

171-183

ISBN

978-1-62703-151-6

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Humana Press

Place of publication

United States of America

Extent

18

Rights statement

Copyright 2013 Springer Science+ Business Media, LLC (Information transferred from record 84137, which was the same paper but which was originally entered as an A1, and which has been deleted. HW 28/2/14)

Repository Status

  • Restricted

Socio-economic Objectives

Environmentally sustainable plant production not elsewhere classified

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    University Of Tasmania

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