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Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes Is Not A Hallmark of Age-Related Macular Degeneration

Citation

Oliver, VF and Franchina, M and Jaffe, AE and Branham, KE and Othman, M and Heckenlively, JR and Swaroop, A and Campochiaro, B and Vote, BJ and Craig, JE and Saffery, R and Mackey, DA and Qian, J and Zack, DJ and Hewitt, AW and Merbs, SL, Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes Is Not A Hallmark of Age-Related Macular Degeneration, Cell Reports, 5, (6) pp. 1527-1535. ISSN 2211-1247 (2013) [Refereed Article]


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Copyright 2013 The authors

DOI: doi:10.1016/j.celrep.2013.11.042

Abstract

Age-related macular degeneration (AMD) is a leading cause of visual impairment worldwide. Aberrant DNA methylation within the promoter of IL17RC in peripheral blood mononuclear cells has recently been reported in AMD. To validate this association, we examined DNA methylation of the IL17RC promoter in peripheral blood. First, we used Illumina Human Methylation450 Bead Arrays, a widely accepted platform for measuring global DNA methylation. Second, methylation status at multiple sites within the IL17RC promoter was determined by bisulfite pyrosequencing in two cohorts. Third, a methylation-sensitive quantitative PCR-based assay was performed on a subset of samples. In contrast to previous findings, we did not find evidence of differential methylation between AMD cases and age-matched controls. We conclude that hypomethylation within the IL17RC gene promoter in peripheral blood is not suitable for use as a clinical biomarker of AMD. This study highlights the need for considerable replication of epigenetic association studies prior to clinical application.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Ophthalmology and Optometry
Research Field:Ophthalmology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Hearing, Vision, Speech and Their Disorders
Author:Vote, BJ (Dr Brendan Vote)
Author:Mackey, DA (Professor David Mackey)
Author:Hewitt, AW (Dr Alex Hewitt)
ID Code:89174
Year Published:2013
Web of Science® Times Cited:17
Deposited By:Medicine (Discipline)
Deposited On:2014-02-26
Last Modified:2017-11-07
Downloads:310 View Download Statistics

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