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Specific determination of intact cisplatin and monohydrated cisplatin in human plasma and culture medium ultrafiltrates using HPLC on-line with inductively coupled plasma mass spectrometry

Citation

Bell, DN and Liu, JJ and Tingle, MD and McKeage, MJ, Specific determination of intact cisplatin and monohydrated cisplatin in human plasma and culture medium ultrafiltrates using HPLC on-line with inductively coupled plasma mass spectrometry, Journal of Chromatography B, 837, (1-2) pp. 29-34. ISSN 1873-376X (2006) [Refereed Article]

Copyright Statement

Copyright 2006 Elsevier

DOI: doi:10.1016/j.jchromb.2006.03.063

Abstract

Abstract We have developed a specific assay for cisplatin in human plasma ultrafiltrate (PUF) and cell culture medium ultrafiltrate (MUF) using HPLC on-line with inductively coupled plasma mass spectrometry (ICP-MS). Separation of cisplatin (6 min) and monohydrated cisplatin (12 min) was achieved using a Bondapak C18 column (Waters) and a mobile phase (0.075mM sodium dodecyl sulfate and 3% methanol, adjusted to pH 2.5 with triflic acid) pumped at a flow rate of 0.5 mL/min. The analytes were detected with little background interference by ICP-MS monitoring of platinum masses (m/z 194/195). Calibration curves were linear over three orders of magnitude (0.05–8 M) and the limit of quantitation was 0.1M. Intra- and inter-assay accuracy (range 91.6–113%) and precision (range 1.00–12.3%) were acceptable for PUF and MUF. The method was applied to determining cisplatin during ex vivo incubation of the drug in whole human blood at 37 ◦C. In conclusion, a specific, sensitive and reliable HPLC–ICP-MS assay has been established for determining intact cisplatin in PUF and MUF.

Item Details

Item Type:Refereed Article
Keywords:cisplatin, HPLC-ICP-MS, human plasma
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmaceutical Sciences
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Liu, JJ (Dr Johnson Liu)
ID Code:89130
Year Published:2006
Web of Science® Times Cited:24
Deposited By:Pharmacy
Deposited On:2014-02-25
Last Modified:2014-08-04
Downloads:0

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