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Electrokinetic removal of charged species from small sample volumes


Quirino, JP, Electrokinetic removal of charged species from small sample volumes, Journal of Chromatography A, 1299 pp. 131-135. ISSN 0021-9673 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2013.05.055


Cationic or anionic molecules can be selectively injected from an aqueous mixture of charged and neutral species into a capillary by electrophoresis. Under stacking conditions, this selective field enhanced sample injection was studied here as a potential sample preparation technique for the clean-up or purification of microliter samples. As a simple demonstration, cationic small molecules were removed or extracted from a 100 μL sample mixture using a commercial capillary electrophoresis instrument. The extraction was monitored using an on-line UV detector. Results from the micellar electrokinetic chromatography analysis of treated and untreated sample revealed significant reduction of cationic species in the treated sample. The technique is essentially a sequence of field enhanced sample injection experiments in capillary electrophoresis.

Item Details

Item Type:Refereed Article
Keywords:sample purification, solventless sample preparation, microliter sample, field enhanced sample injection, capillary electrophoresis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:89030
Year Published:2013
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-02-24
Last Modified:2014-06-06

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