Quirino, JP, Electrokinetic removal of charged species from small sample volumes, Journal of Chromatography A, 1299 pp. 131-135. ISSN 0021-9673 (2013) [Refereed Article]
Copyright © 2013 Elsevier B.V.
Cationic or anionic molecules can be selectively injected from an aqueous mixture of charged and neutral species into a capillary by electrophoresis. Under stacking conditions, this selective field enhanced sample injection was studied here as a potential sample preparation technique for the clean-up or purification of microliter samples. As a simple demonstration, cationic small molecules were removed or extracted from a 100 μL sample mixture using a commercial capillary electrophoresis instrument. The extraction was monitored using an on-line UV detector. Results from the micellar electrokinetic chromatography analysis of treated and untreated sample revealed significant reduction of cationic species in the treated sample. The technique is essentially a sequence of field enhanced sample injection experiments in capillary electrophoresis.
|Item Type:||Refereed Article|
|Keywords:||sample purification, solventless sample preparation, microliter sample, field enhanced sample injection, capillary electrophoresis|
|Research Division:||Chemical Sciences|
|Research Group:||Analytical chemistry|
|Research Field:||Separation science|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding knowledge|
|Objective Field:||Expanding knowledge in the chemical sciences|
|UTAS Author:||Quirino, JP (Associate Professor Lito Quirino)|
|Funding Support:||Australian Research Council (FT100100213)|
|Web of Science® Times Cited:||8|
|Deposited By:||Austn Centre for Research in Separation Science|
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