eCite Digital Repository
GLP-1 increases microvascular recruitment but not glucose uptake in human and rat skeletal muscle
Citation
Sjoberg, KA and Holst, JJ and Rattigan, S and Richter, EA and Kiens, B, GLP-1 increases microvascular recruitment but not glucose uptake in human and rat skeletal muscle, American Journal of Physiology: Endocrinology and Metabolism, 306, (4) pp. E355-E362. ISSN 0193-1849 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 American Physiological Society
DOI: doi:10.1152/ajpendo.00283.2013
Abstract
The insulinotropic gut hormone glucagon-like peptide-1 (GLP-1) has been proposed to have effects on vascular function and glucose disposal. However, whether GLP-1 is able to increase microvascular recruitment (MVR) in humans has not been investigated. GLP-1 was infused in the femoral artery in overnight-fasted, healthy young men. Microvascular recruitment was measured with real-time contrast-enhanced ultrasound and leg glucose uptake by the leg balance technique with and without inhibition of the insulinotropic response of GLP-1 by coinfusion of octreotide. As a positive control, MVR and leg glucose uptake were measured during a hyperinsulinemic-euglycemic clamp. Infusion of GLP-1 caused a rapid increase (P < 0.05) of 20 ± 12% (mean ± SE) in MVR in the vastus lateralis muscle of the infused leg after 5 min, and MVR further increased to 60 ± 8% above preinfusion levels by 60 min infusion. The effect was slightly slower but similar in magnitude in the noninfused contralateral leg, in which GLP-1 concentration was within the physiological range. Octreotide infusion did not prevent the GLP-1-induced increase in MVR. GLP-1 infusion did not increase leg glucose uptake with or without octreotide coinfusion. GLP-1 infusion in rats increased MVR by 28% (P < 0.05) but did not increase muscle glucose uptake. During the hyperinsulinemic clamp, MVR increased ∼40%, and leg glucose uptake increased 35-fold. It is concluded that GLP-1 in physiological concentrations causes a rapid insulin-independent increase in muscle MVR but does not affect muscle glucose uptake.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | skeletal muscle, microcirculation, glucose uptake, GLP-1 |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Clinical sciences |
| Research Field: | Endocrinology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Rattigan, S (Professor Stephen Rattigan) |
| ID Code: | 88788 |
| Year Published: | 2014 |
| Funding Support: | National Health and Medical Research Council (490034) |
| Web of Science® Times Cited: | 46 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2014-02-17 |
| Last Modified: | 2017-11-02 |
| Downloads: | 0 |
Repository Staff Only: item control page