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Comparison of thraustochytrids Aurantiochytrium sp., Schizochytrium sp., Thraustochytrium sp., and Ulkenia sp. for production of biodiesel, long-chain omega-3 oils, and exopolysaccharide

Citation

Lee Chang, KJ and Nichols, CM and Blackburn, SI and Dunstan, GA and Koutoulis, A and Nichols, PD, Comparison of thraustochytrids Aurantiochytrium sp., Schizochytrium sp., Thraustochytrium sp., and Ulkenia sp. for production of biodiesel, long-chain omega-3 oils, and exopolysaccharide, Marine Biotechnology, 16, (4) pp. 396-411. ISSN 1436-2228 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer Science+Business Media New York

DOI: doi:10.1007/s10126-014-9560-5

Abstract

Heterotrophic growth of thraustochytrids has potential in coproducing biodiesel for transportation, as well as producing a feedstock for omega-3 long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA), especially docosahexaenoic acid (DHA) for use in nutraceuticals. In this study, we compared eight new endemic Australian thraustochytrid strains from the genera Aurantiochytrium, Schizochytrium, Thraustochytrium, and Ulkenia for the synthesis of exopolysaccharide (EPS), in addition to biodiesel and LCPUFA. Aurantiochytrium sp. strains readily utilized glucose for biomass production, and increasing glucose from 2 to 4 % w/v of the culture medium resulted in increased biomass yield by an average factor of 1.7. Ulkenia sp. strain TC 010 and Thraustochytrium sp. strain TC 033 did not utilize glucose, while Schizochytrium sp. strain TC 002 utilized less than half the glucose available by day 14, and Thraustochytrium sp. strain TC 004 utilized glucose at 4 % w/v but not 2 % w/v of the culture suggesting a threshold requirement between these values. Across all strains, increasing glucose from 2 to 4 % w/v of the culture medium resulted in increased total fatty acid methyl ester content by an average factor of 1.9. Despite an increasing literature demonstrating the capacity of thraustochytrids for DHA synthesis, the production of EPS from these organisms is not well documented. A broad range of EPS yields was observed. The maximum yield of EPS was observed for Schizochytrium sp. strain TC 002 (299 mg/L). High biomass-producing strains that also have high lipid and high EPS yield may be better candidates for commercial production of biofuels and other coproducts.

Item Details

Item Type:Refereed Article
Keywords:Labyrinthulomycetes, biodiesel, docosahexaenoic acid, exopolysaccharide
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lee Chang, KJ (Mr Kim Lee Chang)
Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:88573
Year Published:2014
Web of Science® Times Cited:8
Deposited By:Plant Science
Deposited On:2014-02-07
Last Modified:2017-01-24
Downloads:1 View Download Statistics

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