A dry film resist (DFR) chip compatible with the Agilent Bioanalyzer 2100 was designed and fabricated for use in the analysis of lactate in serum by chip isotachophoresis (ITP). The Agilent Bioanalyzer 2100 is a commercially available field deployable analytical instrument originally developed for the elec-trophoretic analysis of DNA, RNA and proteins. The DFR chip was designed for the ITP separation of lactate in human serum within 1 min and was made compatible with the Bioanalyzer after packaging in the plastic caddies normally used for the DNA chips. A 20-fold improvement in sensitivity was obtained for the DFR chips in comparison with the standard chips used in earlier work. The limit of detection and limit of quantification for lactate were 24 μM and 80 μM, respectively. This new approach enables the use of commercial platforms like the Agilent Bioanalyzer for new applications including the analysis of small molecules.

Item Type:	Refereed Article
Keywords:	electrophoresis, isotachophoresis, microfluidics, fluorescence, indirect detection, serum
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Smejkal, P (Dr Petr Smejkal)
Author:	Breadmore, MC (Professor Michael Breadmore)
Author:	Guijt, RM (Dr Rosanne Guijt)
Author:	Macka, M (Professor Mirek Macka)
ID Code:	88434
Year Published:	2013
Funding Support:	Australian Research Council (DP0984745)
Web of Science® Times Cited:	12
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2014-02-03
Last Modified:	2014-08-11
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