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Sieving polymer synthesis by reversible addition fragmentation chain transfer polymerization


Nai, YH and Jones, RC and Breadmore, MC, Sieving polymer synthesis by reversible addition fragmentation chain transfer polymerization, Electrophoresis, 34, (22-23) pp. 3189-3197. ISSN 0173-0835 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201300288


Replaceable sieving polymers are the fundamental component for high-resolution nucleic acids separation in CE. The choice of polymer and its physical properties play significant roles in influencing separation performance. Recently, reversible addition fragmentation chain transfer (RAFT) polymerization has been shown to be a versatile polymerization technique capable of yielding well-defined polymers previously unattainable by conventional free-radical polymerization. In this study, a high molecular weight poly-(N,N-dimethylacrylamide) (PDMA) at 765000 gmol-1 with a polydispersity index of 1.55 was successfully synthesized with the use of chain transfer agent-2-propionic acidyl butyl trithiocarbonate in a multistep sequential RAFT polymerization approach. This study represents the first demonstration of RAFT polymerization for synthesizing polymers with the molecular weight range suitable for high-resolution DNA separation in sieving electrophoresis. Adjustment of pH in the reaction was found to be crucial for the successful RAFT polymerization of high molecular weight polymer as the buffered condition minimizes the effect of hydrolysis and aminolysis commonly associated with trithiocarbonate chain transfer agents. The separation efficiency of 2-propionic acidyl butyl trithiocarbonate PDMA was found to have marginally superior separation performance compared to a commercial PDMA formulation, POPTM-CAP, of similar molecular weight range.

Item Details

Item Type:Refereed Article
Keywords:electrophoresis, RNA, DNA separation, RAFT polymerization, sieving polymer
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Nai, YH (Mr Ryan Nai)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:88425
Year Published:2013
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:3
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-02-03
Last Modified:2014-05-09

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