File(s) under permanent embargo
Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking
journal contribution
posted on 2023-05-17, 21:37 authored by Lu, Y, Bai, H, Kong, C, Zhong, H, Michael BreadmoreMichael BreadmoreA method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
34Issue
24Pagination
3326-3332ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley - V C H Verlag GmbH & Co. KGaAPlace of publication
GermanyRights statement
Copyright 2013 Wiley-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted