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Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking

Citation

Lu, Y and Bai, H and Kong, C and Zhong, H and Breadmore, MC, Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking, Electrophoresis, 34, (24) pp. 3326-3332. ISSN 0173-0835 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201300402

Abstract

A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.

Item Details

Item Type:Refereed Article
Keywords:electrophoresis, flavonoids, acid barrage stacking (ABS), brazilin, CE, protosappanin B
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:88424
Year Published:2013
Funding Support:Australian Research Council (DP0984745)
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-02-03
Last Modified:2015-01-21
Downloads:0

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