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Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking
Citation
Lu, Y and Bai, H and Kong, C and Zhong, H and Breadmore, MC, Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking, Electrophoresis, 34, (24) pp. 3326-3332. ISSN 0173-0835 (2013) [Refereed Article]
Copyright Statement
Copyright 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: doi:10.1002/elps.201300402
Abstract
A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.
Item Details
Item Type: | Refereed Article |
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Keywords: | electrophoresis, flavonoids, acid barrage stacking (ABS), brazilin, CE, protosappanin B |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
ID Code: | 88424 |
Year Published: | 2013 |
Funding Support: | Australian Research Council (DP0984745) |
Web of Science® Times Cited: | 15 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2014-02-03 |
Last Modified: | 2015-01-21 |
Downloads: | 0 |
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