A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.

Item Type:	Refereed Article
Keywords:	electrophoresis, flavonoids, acid barrage stacking (ABS), brazilin, CE, protosappanin B
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
UTAS Author:	Breadmore, MC (Professor Michael Breadmore)
ID Code:	88424
Year Published:	2013
Funding Support:	Australian Research Council (DP0984745)
Web of Science® Times Cited:	9
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2014-02-03
Last Modified:	2015-01-21
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