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Approaches to Enhancing the Sensitivity of Carbohydrate Separations in Capillary Electrophoresis
Electrophoresis in both capillaries (CE) and microchips (ME) is an extremely powerful liquid phase-separation technique that is indispensable for the separation of carbohydrates. It is capable of separating both small mono- and disaccharides, through to more complex oligo- and polysaccharides, with high resolution, but as with all CE and ME separations, the detection limits are often inferior to those that can be achieved with liquid chromatographic methods. One avenue to address this is to use an on-line concentration strategy. Various approaches have been developed over the past 20 years, and this chapter will highlight their application to improve the sensitivity of carbohydrate separations in both CE and ME.
Funding
Australian Research Council
History
Publication title
Capillary Electrophoresis of Biomolecules: Methods and ProtocolsVolume
984Editors
N Volpi and F MaccariPagination
27-43ISBN
978-1-62703-295-7Department/School
School of Natural SciencesPublisher
Humana PressPlace of publication
New York, USAExtent
25Rights statement
Copyright 2013 Springer Science+Business MediaRepository Status
- Restricted