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Novel gammaherpesviruses in North American domestic cats, bobcats and pumas: identification, prevalence and risk factors

Citation

Troyer, RM and Beatty, JA and Stutzman-Rodriguez, KR and Carver, S and Lozano, CC and Lee, JS and Lappin, MR and Riley, SPD and Serieys, LEK and Logan, KA and Sweanor, LL and Boyce, WM and Vickers, TW and McBride, R and Crooks, KR and Lewis, JS and Cunningham, MW and Rovnak, J and Quackenbush, SL and VandeWoude, S, Novel gammaherpesviruses in North American domestic cats, bobcats and pumas: identification, prevalence and risk factors, Journal of Virology, 88, (8) pp. 3914-3924. ISSN 0022-538X (2014) [Refereed Article]


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Copyright Statement

Copyright 2014 American Society for Microbiology

DOI: doi:10.1128/JVI.03405-13

Abstract

Gammaherpesviruses (GHVs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. To identify felid GHVs, we screened domestic cat (Felis catus), bobcat (Lynx rufus) and puma (Puma concolor) blood cell DNA samples from California, Colorado and Florida using a degenerate pan-GHV PCR. Additional pan-GHV and long-distance PCRs were used to sequence a contiguous 3.4 kb region of each putative virus species including partial glycoprotein B and DNA polymerase genes. We identified three novel GHVs, each present predominantly in one felid species: Felis catus GHV 1 (FcaGHV1) in domestic cats, Lynx rufus GHV 1 (LruGHV1) in bobcats, and Puma concolor GHV 1 (PcoGHV1) in pumas. To estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and screened additional DNA samples from all three species (n = 282). FcaGHV1 was detected in 16% of domestic cats across all study sites. LruGHV1 was detected in 47% of bobcats and 13% of pumas across all study sites, suggesting relatively common interspecific transmission. PcoGHV1 was detected in 6% of pumas, all from a specific region of Southern California. The risk of infection for each host varied with geographic location. Age was a positive risk factor for bobcat LruGHV1 infection, and age and being male were risk factors for domestic cat FcaGHV1 infection. Further characterization of these viruses may have significant health implications for domestic cats and may aid studies of free-ranging felid ecology.

Item Details

Item Type:Refereed Article
Keywords:gammaherpesvirus, epidemiology, disease ecology
Research Division:Biological Sciences
Research Group:Evolutionary Biology
Research Field:Host-Parasite Interactions
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Disease Distribution and Transmission (incl. Surveillance and Response)
Author:Carver, S (Dr Scott Carver)
ID Code:88354
Year Published:2014
Web of Science® Times Cited:18
Deposited By:Zoology
Deposited On:2014-01-29
Last Modified:2017-11-01
Downloads:135 View Download Statistics

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