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Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar

Citation

Nowak, BF and Cadoret, K and Feist, SW and Bean, TP, Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar, Journal of Fish Biology, 83, (5) pp. 1459-1467. ISSN 1095-8649 (2013) [Refereed Article]

Copyright Statement

2013 Crown Copyright; Copyright 2013 The Fisheries Society of the British Isles

DOI: doi:10.1111/jfb.12235

Abstract

Laser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.

Item Details

Item Type:Refereed Article
Keywords:anadromous; anterior gradient protein; microdissection; qPCR; salmonid
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Cadoret, K (Miss Karine Cadoret)
ID Code:88000
Year Published:2013
Web of Science® Times Cited:6
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-01-06
Last Modified:2017-07-18
Downloads:0

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