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Laser-capture dissection and immunohistochemistry reveals chloride and mucous-cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar
journal contribution
posted on 2023-05-17, 21:08 authored by Barbara NowakBarbara Nowak, Karine CadoretKarine Cadoret, Feist, SW, Bean, TPLaser-capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar, with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.
History
Publication title
Journal of Fish BiologyVolume
83Issue
5Pagination
1459-1467ISSN
1095-8649Department/School
Institute for Marine and Antarctic StudiesPublisher
WileyPlace of publication
Malden MA USARights statement
2013 Crown Copyright; Copyright 2013 The Fisheries Society of the British IslesRepository Status
- Restricted