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Development and experimental application of an HPLC procedure for the determination of capsaicin and dihydrocapsaicin in serum samples from human subjects

Citation

Hartley, TF and Stevens, BJ and Ahuja, KDK and Ball, MJ, Development and experimental application of an HPLC procedure for the determination of capsaicin and dihydrocapsaicin in serum samples from human subjects, Proceedings of the 40th International Symposium on High Performance Liquid Phase Separations and Related Techniques, 1-21 November 2013, Hobart, Tasmania, pp. 26. (2013) [Conference Extract]

Abstract

Total capsaicins were extracted from 2 mL aliquots of serum or plasma using methyl-isobutyl ketone, evaporation of the extract to dryness and reconstitution with 200 ÁL of acetonitrile. The HPLC mobile phase was 40:60 water:acetonitrile pumped at 0.5ml/min through an Phenomenex Luna C18s column. The absorbance of the eluent was monitored at 205 nm. Standardisation used a known mixture of pure capsaicin and dihydrocapsaicin. Accuracies were 98.9% and 100.6% for capsaicin and dihydrocapsaicin respectively. Inter batch reproducibility for both was 15%. The limits of detection were 2.6 and 3.8 ng/mL for capsaicin and dihydrocapsaicin respectively. Analyses of sera obtained previously from human subjects who had eaten chilli containing meals showed that in those that absorbed capsaicins (N=30) then the median, mean and SD of their serum capsaicin were : 13.4 ng/mL, 18.9 ng/mL and 16.3 ng/mL. The corresponding data for those sera (N=13) that had measurable levels of dihydrocapsaicin were : 6.9 ng/mL, 7.5 ng/mL and 3.6 ng/mL. This procedure was deemed suitable for use in prospective studies of the metabolism of orally ingested chilli.

Item Details

Item Type:Conference Extract
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Chemistry not elsewhere classified
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Nutrition
Author:Hartley, TF (Dr Thomas Hartley)
Author:Stevens, BJ (Dr Brian Stevens)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Ball, MJ (Professor Madeleine Ball)
ID Code:87967
Year Published:2013
Deposited By:Health Sciences A
Deposited On:2013-12-20
Last Modified:2014-05-21
Downloads:0

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