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Denervation of the olfactory bulb leads to decreased Aβ plaque load in a transgenic mouse model of Alzheimer's disease

Citation

Bibari, O and Lee, S and Dickson, TC and Mitew, S and Vickers, JC and Chuah, MI, Denervation of the olfactory bulb leads to decreased Aβ plaque load in a transgenic mouse model of Alzheimer's disease, Current Alzheimer Research, 10, (7) pp. 688-696. ISSN 1567-2050 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Bentham Science Publishers

DOI: doi:10.2174/15672050113109990147

Abstract

The aggregation of beta-amyloid (Aβ) into plaques in the extracellular compartment of the brain is a pathological hallmark of Alzheimer's disease (AD). Although the pathways for misprocessing of Aβ leading to plaque formation are not well understood, they may be related to synapse turnover and neuron activity. In this study, we have utilised transgenic mice co-expressing mutations in the amyloid precursor protein and presenilin 1 genes (APP/PS1) to determine how long-term denervation of the olfactory bulb, a CNS area affected early by AD-like pathology, may affect Aβ plaque formation. The olfactory bulb of pre-symptomatic mice was denervated by ablating the olfactory epithelium unilaterally with Triton X-100 solution. Mice were subjected to nasal washes for a total of 4 or 8 times, at 3-week intervals either with 1% Triton X-100 solution or phosphate buffered saline (sham denervation). Denervation of the olfactory bulb resulted in a statistically significant (p<0.05) decrease in amyloid plaque load in the ipsilateral olfactory bulb, and bilaterally also in the neocortex and hippocampus at 8-9 months age. Amyloid precursor protein was predominantly expressed by mitral cells in the olfactory bulb, which are normally postsynaptic to olfactory axons. The number of APP positive mitral cells was significantly increased in the denervated olfactory bulb of wild type but not of the APP/PS1 mice, which consistently showed high immunoreactivity for APP pre- and post-denervation. In summary, our results show that Aβ plaque deposition in the central nervous system can be modified transsynaptically by deafferentation.

Item Details

Item Type:Refereed Article
Keywords:Alzheimer's disease, amyloid plaque load, amyloid precursor protein, denervation, olfactory bulb
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Bibari, O (Dr Olivier Bibari)
Author:Lee, S (Mr Siak Lee)
Author:Dickson, TC (Professor Tracey Dickson)
Author:Mitew, S (Mr Stanislaw Mitew)
Author:Vickers, JC (Professor James Vickers)
Author:Chuah, MI (Dr Inn Chuah)
ID Code:87827
Year Published:2013
Web of Science® Times Cited:3
Deposited By:Menzies Institute for Medical Research
Deposited On:2013-12-10
Last Modified:2017-11-06
Downloads:0

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