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Cortical murine neurons lacking the neurofilament light chain protein have an attenuated response to injury in vitro
Citation
Blizzard, CA and King, AE and Vickers, J and Dickson, T, Cortical murine neurons lacking the neurofilament light chain protein have an attenuated response to injury in vitro, Journal of Neurotrauma, 30, (22) pp. 1908-1918. ISSN 0897-7151 (2013) [Refereed Article]
Copyright Statement
Copyright 2013 Mary Ann Liebert
DOI: doi:10.1089/neu.2013.2850
Abstract
Abstract Neurofilaments (NFs) have been proposed to have a significant role in attempted axonal regeneration following a variety of forms of injury. The NF triplet proteins of the central nervous system are comprised of light (NF-L), medium (NF-M) and heavy (NF-H) chains and are part of the type IV intermediate filament family. We sought to define the role of NF-L in the neuronal response to trauma and regeneration by examining the effect of total absence of the NF-L protein on neuronal maturation and response to axotomy. This study utilized an in vitro model comprising relatively mature cortical murine neurons derived from either wild-type embryonic (E15) mice or mice with a genetic knockout of NF-L (NF-L KO). Whilst NF-L KO neurons developed to relative maturity at a comparable rate to wild-type control neurons, NF-L KO neurons demonstrated relatively increased expression of α-internexin and decreased expression of NF-M. Further, we demonstrate that α-internexin co-immunoprecipitates with the NF binding protein NDel1 in NFL-KO cortical neurons in vitro. Following localized axotomy, NF-L KO neurons demonstrated reduced amyloid precursor protein accumulation in damaged neurites as well as a significant reduction in the number of axons regenerating (4.79+/-0.58 sprouts) in comparison to control preparations (10.47+/-1.11 sprouts) (p<0.05). These studies indicate that NFs comprising NF-L have a dynamic role in the reactive and regenerative changes in axons following injury.
Item Details
Item Type: | Refereed Article |
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Keywords: | axotomy, cytoskeleton, neurofilaments, sprouting, trauma |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Neurosciences |
Research Field: | Central nervous system |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Blizzard, CA (Dr Catherine Blizzard) |
UTAS Author: | King, AE (Professor Anna King) |
UTAS Author: | Vickers, J (Professor James Vickers) |
UTAS Author: | Dickson, T (Professor Tracey Dickson) |
ID Code: | 87538 |
Year Published: | 2013 |
Web of Science® Times Cited: | 7 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2013-11-21 |
Last Modified: | 2017-11-06 |
Downloads: | 0 |
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