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Domestic cat microsphere immunoassays: Detection of antibodies during feline immunodeficiency virus infection

Citation

Wood, BA and Carver, S and Troyer, RM and Elder, JH and VandeWoude, S, Domestic cat microsphere immunoassays: Detection of antibodies during feline immunodeficiency virus infection, Journal of Immunological Methods, 396 pp. 74-86. ISSN 0022-1759 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V

DOI: doi:10.1016/j.jim.2013.08.001

Abstract

Microsphere immunoassays (MIAs) allow rapid and accurate evaluation of multiple analytes simultaneously within a biological sample. Here we describe the development and validation of domestic cat-specific MIAs for a) the quantification of total IgG and IgA levels in plasma, and b) the detection of IgG and IgA antibodies to feline immunodeficiency virus (FIV) capsid (CA) and surface (SU) proteins, and feline CD134 in plasma. These assays were used to examine the temporal antibody response of domestic cats infected with apathogenic and pathogenic FIVs, and domestic cats infected with parental and chimeric FIVs of varying pathogenicity. The results from these studies demonstrated that a) total IgG antibodies increase over time after infection; b) α-CA and α-SU IgG antibodies are detectable between 9 and 28 days post-infection and increase over time, and these antibodies combined represent a fraction (1.8 to 21.8%) of the total IgG increase due to infection; c) measurable α-CD134 IgG antibody levels vary among individuals and over time, and are not strongly correlated with viral load; d) circulating IgA antibodies, in general, do not increase during the early stage of infection; and e) total IgG, and α-CA and α-SU IgG antibody kinetics and levels vary with FIV viral strain/pathogenicity. The MIAs described here could be used to screen domestic cats for FIV infection, and to evaluate the FIV-specific or total antibody response elicited by various FIV strains/other diseases.

Item Details

Item Type:Refereed Article
Keywords:domestic cat, feline Immunodefiency virus, microsphere immunoassay, antibody detection, multiplexing
Research Division:Biological Sciences
Research Group:Evolutionary Biology
Research Field:Host-Parasite Interactions
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Disease Distribution and Transmission (incl. Surveillance and Response)
Author:Carver, S (Dr Scott Carver)
ID Code:87001
Year Published:2013
Web of Science® Times Cited:5
Deposited By:Zoology
Deposited On:2013-11-05
Last Modified:2017-11-03
Downloads:1 View Download Statistics

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