File(s) under permanent embargo
Ion-exclusion chromatography of aliphatic carboxylic acids on an unmodified silica gel column
journal contribution
posted on 2023-05-16, 10:20 authored by Ohta, K, Tanaka, K, Paul HaddadPaul HaddadIn order to demonstrate the effectiveness of a silica gel column in ion-exclusion chromatography, the elution behavior of aliphatic carboxylic acids was investigated on an unmodified silica gel column having cation-exchange characteristics. When water was used as an eluent, the elution order of the carboxylic acids was dependent on the pKa and the hydrophobicity, but the resolution between strong acids, e.g. HNO3, and carboxylic acids was not satisfactory. Therefore, the composition of eluents containing sulfuric acid and organic modifiers was investigated for the separation of HNO3 and C1–C10 aliphatic carboxylic acids. When 0.5 mM sulfuric acid containing 0.05% heptanol was used as an eluent, an excellent ion-exclusion chromatographic separation was achieved in 30 min using UV-detection at 220 nm.
History
Publication title
Journal of Chromatography AVolume
739Issue
1-2Pagination
359-365ISSN
0021-9673Department/School
School of Natural SciencesPublisher
Elsevier Science BvPlace of publication
The NetherlandsRights statement
The definitive version is available at http://www.sciencedirect.comRepository Status
- Restricted