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Salinity-induced calcium signaling and root adaptation in Arabidopsis require the calcium regulatory protein annexin1

journal contribution
posted on 2023-05-17, 20:01 authored by Laohavisit, A, Richards, SL, Svetlana ShabalaSvetlana Shabala, Chen, C, Colaco, RDDR, Swarbreck, SM, Shaw, E, Dark, A, Sergey ShabalaSergey Shabala, Shang, Z, Davies, JM
Salinity (NaCl) stress impairs plant growth and inflicts severe crop losses. In roots, increasing extracellular NaCl causes Ca2+ influx to elevate cytosolic free Ca2+ ([Ca2+]cyt) as a second messenger for adaptive signaling. Amplification of the signal involves plasma membrane reduced nicotinamide adenine dinucleotide phosphate oxidase activation, with the resultant reactive oxygen species triggering Ca2+ influx. The genetic identities of the Ca2+-permeable channels involved in generating the [Ca2+]cyt signal are unknown. Potential candidates in the model plant Arabidopsis (Arabidopsis thaliana) include annexin1 (AtANN1). Here, luminescent detection of [Ca2+]cyt showed that AtANN1 responds to high extracellular NaCl by mediating reactive oxygen species-activated Ca2+ influx across the plasma membrane of root epidermal protoplasts. Electrophysiological analysis revealed that root epidermal plasma membrane Ca2+ influx currents activated by NaCl are absent from the Atann1 loss-of-function mutant. Both adaptive signaling and salt-responsive production of secondary roots are impaired in the loss-of-function mutant, thus identifying AtANN1 as a key component of root cell adaptation to salinity.

History

Publication title

Plant Physiology

Volume

163

Pagination

253-262

ISSN

0032-0889

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Amer Soc Plant Biologists

Place of publication

15501 Monona Drive, Rockville, USA, Md, 20855

Rights statement

Copyright 2013 American Society of Plant Biologists. All Rights Reserved

Repository Status

  • Restricted

Socio-economic Objectives

Grains and seeds not elsewhere classified

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