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Salinity-induced calcium signaling and root adaptation in Arabidopsis require the calcium regulatory protein annexin1

Citation

Laohavisit, A and Richards, SL and Shabala, L and Chen, C and Colaco, RDDR and Swarbreck, SM and Shaw, E and Dark, A and Shabala, S and Shang, Z and Davies, JM, Salinity-induced calcium signaling and root adaptation in Arabidopsis require the calcium regulatory protein annexin1, Plant Physiology, 163, (1) pp. 253-262. ISSN 0032-0889 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 American Society of Plant Biologists. All Rights Reserved

DOI: doi:10.1104/pp.113.217810

Abstract

Salinity (NaCl) stress impairs plant growth and inflicts severe crop losses. In roots, increasing extracellular NaCl causes Ca2+ influx to elevate cytosolic free Ca2+ ([Ca2+]cyt) as a second messenger for adaptive signaling. Amplification of the signal involves plasma membrane reduced nicotinamide adenine dinucleotide phosphate oxidase activation, with the resultant reactive oxygen species triggering Ca2+ influx. The genetic identities of the Ca2+-permeable channels involved in generating the [Ca2+]cyt signal are unknown. Potential candidates in the model plant Arabidopsis (Arabidopsis thaliana) include annexin1 (AtANN1). Here, luminescent detection of [Ca2+]cyt showed that AtANN1 responds to high extracellular NaCl by mediating reactive oxygen species-activated Ca2+ influx across the plasma membrane of root epidermal protoplasts. Electrophysiological analysis revealed that root epidermal plasma membrane Ca2+ influx currents activated by NaCl are absent from the Atann1 loss-of-function mutant. Both adaptive signaling and salt-responsive production of secondary roots are impaired in the loss-of-function mutant, thus identifying AtANN1 as a key component of root cell adaptation to salinity.

Item Details

Item Type:Refereed Article
Keywords:root adaptation, Arabidopsis, calcium, annexin1
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Summer Grains and Oilseeds
Objective Field:Summer Grains and Oilseeds not elsewhere classified
Author:Shabala, L (Dr Lana Shabala)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:86961
Year Published:2013
Web of Science® Times Cited:27
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-11-04
Last Modified:2014-05-28
Downloads:0

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