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Monolithic cryopolymers with embedded nanoparticles. II. Capillary liquid chromatography of proteins using charged embedded nanoparticles

Citation

Arrua, RD and Haddad, PR and Hilder, EF, Monolithic cryopolymers with embedded nanoparticles. II. Capillary liquid chromatography of proteins using charged embedded nanoparticles, Journal of Chromatography A, 1311 pp. 121-126. ISSN 0021-9673 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2013.08.077

Abstract

The preparation of composite monolithic cryopolymers is presented. These novel porous materials were prepared in capillary format at −70C using poly (ethyleneglycol) diacrylate (PEGDA) Mw258 as the single monomer and a mixture of dioxane and water as the porogen. Positively (-NR4+) or negatively (SO3) charged nanoparticles were incorporated within the polymeric structure by direct addition of their suspensions to the polymerisation mixture. In contrast to our previous report using neutral nanoparticles, the trapping of charged nanoparticles is mostly observed at the polymer surface. The incorporation of these nanostructures improved the chromatographic separations of standard proteins under a hydrophobic interaction chromatography (HIC) separation mode. Moreover, the presence of ionic groups on the polymer surface allowed the application of these columns under ion-exchange (IEX) conditions. The results obtained in this work show that the functionalisation of monolithic columns by direct addition of nanoparticles is a good alternative towards the modification of monolithic polymers without altering the polymeric scaffold.

Item Details

Item Type:Refereed Article
Keywords:polymer monoliths, cryopolymers, embedded nanoparticles, proteins
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Arrua, RD (Dr Dario Arrua)
Author:Haddad, PR (Professor Paul Haddad)
Author:Hilder, EF (Professor Emily Hilder)
ID Code:86951
Year Published:2013
Web of Science® Times Cited:12
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-11-04
Last Modified:2014-05-08
Downloads:0

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