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Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)

Citation

Valdenegro Vega, VA and Crosbie, PBB and Vincent, BN and Cain, K and Nowak, BF, Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar), Veterinary Immunology and Immunopathology: An International Journal of Comparative Immunology, 151, (1-2) pp. 113-123. ISSN 0165-2427 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier B.V.

DOI: doi:10.1016/j.vetimm.2012.10.010

Abstract

This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) administeredusingdifferentdeliverystrategies. Fishwereexposedtotheantigens through different routes, andwere givenabooster4weekspostinitial exposure.Bothsystemic and mucosal antibody responses were measured for a period of 12 weeks using an enzymelinkedimmunosorbentassay(ELISA).Onlyfishexposedtobothantigensviaintraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was anincrease inantibody levels inthe tissue supernatantfromskinexplantsobtained12 weeks postimmunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens.In conclusion,IP injection of hapten-antigen inAtlantic salmon was the best delivery route for inducing anantibodyresponse againstthese antigens inthis species.Eventhough IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Valdenegro Vega, VA (Miss Victoria Valdenegro)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Vincent, BN (Miss Benita Vincent)
Author:Cain, K (Associate Professor ken Cain)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:86381
Year Published:2013
Web of Science® Times Cited:18
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-09-11
Last Modified:2015-02-02
Downloads:0

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