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Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L

Citation

Zainathan, SC and Carson, J and Crane, MSJ and Nowak, BF, Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L, Journal of Fish Diseases, 36, (4) pp. 427-436. ISSN 0140-7775 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Blackwell Publishing Ltd

DOI: doi:10.1111/jfd.12012

Abstract

The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine the sensitivity of the collection methods and the effect of pooling of samples for the detection of TSRV. Detection of TSRV in individual samples was as sensitive when organs were sampled compared to swabs, and in pooled samples, organs demonstrated a sensitivity of one 10-fold dilution higher than sampling of pooled swabs. Storage of swabs at 4 C for t = 24 h demonstrated results similar to those at t = 0. Advantages of using swabs as a preferred sample collection method for the detection of TSRV compared to organ samples are evident from these experimental trials.

Item Details

Item Type:Refereed Article
Keywords:Atlantic salmon;organ;swabs;Tasmania;Tasmanian salmon reovirus
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Zainathan, SC (Ms Sandra Zainathan)
Author:Carson, J (Dr Jeremy Carson)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:86380
Year Published:2013
Web of Science® Times Cited:2
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-09-11
Last Modified:2017-07-18
Downloads:0

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