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Effects of exposure to oestrogenic compounds on aromatase gene expression are gender dependent in the rainbowfish, Melanotaenia fluviatilis

Citation

Shanthanagouda, AH and Patil, JG and Nugegoda, D, Effects of exposure to oestrogenic compounds on aromatase gene expression are gender dependent in the rainbowfish, Melanotaenia fluviatilis, Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology, 157, (2) pp. 162-171. ISSN 1878-1659 (2013) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier Inc.

DOI: doi:10.1016/j.cbpc.2012.11.004

Abstract

This study investigated the influence of two endocrine disrupting chemicals (EDCs)—an exogenous oestrogen 17β-estradiol (E2) and the oestrogen mimic 4-n-nonylphenol (NP) on the expression of aromatase transcripts in both sexes of adult Murray river rainbowfish. Reproductively active mature fish were exposed to 1, 3, and 5 μg/L E2 or 100 and 500 μg/L NP for 24, 48, 72 and 96 h. The results show a significant reduction in the expression of cyp19a1a isoform in ovarian tissues with complete inhibition at the higher concentrations (3 and 5 μg/L E2; 500 μg/L NP between 24 and 72 h) and at all concentrations after 96 h. There was no expression of the cyp19a1a isoform in female brain, male brain or testes in any treatment. E2 significantly increased expression of cyp19a1b in female brain except at 5 μg/L after 24 h exposure. In male brain tissue E2 exposure decreased cyp19a1b expression except at 1 and 5 μg/L at 24 h. NP significantly upregulated cyp19a1b in the female brain (except with 500 μg/L at 72 h) and in testes tissues. NP downregulated expression of cyp19a1b in the male brain tissue. Collectively, these observations support the hypothesis that the expression of cyp19a1b is regulated via both positive and negative feedback mechanisms, with differential modulation based on the type and concentration of the exposed oestrogens, duration of exposure, fish tissue and gender of the fish. The results also imply that exogenous oestrogens can have a disruptive effect on the steroidogenic pathway and may lead to effects on sex differentiation, sexual behaviour and reproductive cycles in this fish.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Physiology and Genetics
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments
Author:Patil, JG (Dr Jawahar Patil)
ID Code:86092
Year Published:2013
Web of Science® Times Cited:13
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-08-22
Last Modified:2017-10-31
Downloads:0

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