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Exposure to Estrogenic Chemicals Induces Ectopic Expression of vtg in the Testis of Rainbowfish, Melanotaenia fluviatilis

Citation

Shanthanagouda, AH and Nugegoda, D and Hassell, KL and Patil, JG, Exposure to Estrogenic Chemicals Induces Ectopic Expression of vtg in the Testis of Rainbowfish, Melanotaenia fluviatilis, Bulletin of Environmental Contamination and Toxicology, 91, (4) pp. 438-443. ISSN 0007-4861 (2013) [Refereed Article]

Copyright Statement

Copyright Springer Science+Business Media

DOI: doi:10.1007/s00128-013-1078-4

Abstract

Vitellogenin (Vtg) is the major egg-yolk precursor protein in oviparous organisms normally synthesised only in mature females. In males and juveniles, the vtg gene, although present, is silent, but its hepatic expression may be activated by xenoestrogens. Surprisingly, its induction and potential consequences in non-hepatic tissues remain unexplored. Here we test the hepatic and testicular response of vtg expression in adult male rainbowfish Melanotaenia fluviatilis exposed to either 1, 3, 5 lg/L 17bestradiol or 100, 500 lg/L 4-n-nonylphenol for 24–96 h. Significant increase in the expression level of vtg mRNA in the liver and testes of exposed males was observed. The early (24 h), sensitive and reliable detection of the vtg induction using qPCR demonstrates the assay’s robustness to monitor xenobiotic exposure particularly in smaller fish like rainbowfish, an emerging indicator species. Whilst, the ectopic induction of vtg mRNA in testes suggests a more complex Vtg pathway

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Physiology and Genetics
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments
Author:Patil, JG (Dr Jawahar Patil)
ID Code:86085
Year Published:2013
Web of Science® Times Cited:6
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-08-22
Last Modified:2017-01-11
Downloads:0

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