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Exposure to Estrogenic Chemicals Induces Ectopic Expression of vtg in the Testis of Rainbowfish, Melanotaenia fluviatilis
Citation
Shanthanagouda, AH and Nugegoda, D and Hassell, KL and Patil, JG, Exposure to Estrogenic Chemicals Induces Ectopic Expression of vtg in the Testis of Rainbowfish, Melanotaenia fluviatilis, Bulletin of Environmental Contamination and Toxicology, 91, (4) pp. 438-443. ISSN 0007-4861 (2013) [Refereed Article]
Copyright Statement
Copyright Springer Science+Business Media
DOI: doi:10.1007/s00128-013-1078-4
Abstract
Vitellogenin (Vtg) is the major egg-yolk precursor protein in oviparous organisms normally synthesised
only in mature females. In males and juveniles, the vtg
gene, although present, is silent, but its hepatic expression
may be activated by xenoestrogens. Surprisingly, its
induction and potential consequences in non-hepatic tissues remain unexplored. Here we test the hepatic and testicular response of vtg expression in adult male rainbowfish
Melanotaenia fluviatilis exposed to either 1, 3, 5 lg/L 17bestradiol or 100, 500 lg/L 4-n-nonylphenol for 24–96 h.
Significant increase in the expression level of vtg mRNA in
the liver and testes of exposed males was observed. The
early (24 h), sensitive and reliable detection of the vtg
induction using qPCR demonstrates the assay’s robustness
to monitor xenobiotic exposure particularly in smaller fish
like rainbowfish, an emerging indicator species. Whilst, the
ectopic induction of vtg mRNA in testes suggests a more
complex Vtg pathway
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Fish physiology and genetics |
Objective Division: | Environmental Management |
Objective Group: | Marine systems and management |
Objective Field: | Assessment and management of benthic marine ecosystems |
UTAS Author: | Patil, JG (Dr Jawahar Patil) |
ID Code: | 86085 |
Year Published: | 2013 |
Web of Science® Times Cited: | 7 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2013-08-22 |
Last Modified: | 2018-03-17 |
Downloads: | 0 |
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