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The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury

Citation

Gonzalez, P and Fernandez-Martos, CM and Arenas, E and Rodriguez, FJ, The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury, Journal of Neurotrauma, 30, (10) pp. 806 - 817. ISSN 0897-7151 (2013) [Refereed Article]

DOI: doi:10.1089/neu.2012.2613

Abstract

Wnt proteins play a critical role in central nervous system development and have been implicated in several neuropathologies, including spinal cord injury (SCI). Ryk, an unconventional Wnt receptor, regulates axonal regeneration after SCI, although its expression pattern in this neuropathology remains unclear. Therefore, we sought to define the spatiotemporal and cellular pattern of Ryk expression after a contusive SCI in adult rats using quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemical analysis. Under physiological conditions, Ryk is expressed in neurons, astrocytes, and blood vessels, but not in oligodendrocytes, microglia, NG2+ glial precursor cells, or axonal projections. Following SCI, we observed an increase in Ryk mRNA expression from 24 h post-injury until 7 days post-injury, whereas its protein levels were significantly augmented at 7 and 14 days post-injury. Moreover, the spatial and cellular Ryk expression pattern was altered in the damaged tissue, where this receptor was observed in reactive astrocytes and microglia/macrophages, NG2+ glial precursors, fibronectin+ cells, oligodendrocytes, and axons. In conclusion, we demonstrate that Ryk is expressed in the unlesioned spinal cord and that, after SCI, its spatiotemporal and cellular expression pattern changed dramatically, being expressed in cells involved in the spinal cord response to damage.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:Fernandez-Martos, CM (Dr Carmen Fernandez-Martos)
ID Code:86010
Year Published:2013
Web of Science® Times Cited:7
Deposited By:Medicine (Discipline)
Deposited On:2013-08-20
Last Modified:2014-06-10
Downloads:0

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