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S-resistin inhibits adipocyte differentiation and increases TNFalpha expression and secretion in 3T3-L1 cells

Citation

Fernandez-Martos, CM and del Arco, A and Gallardo, N and Aguado, L and Rodriguez, M and Ros, M and Carrascosa, JM and Andres, A and Arribas, C, S-resistin inhibits adipocyte differentiation and increases TNFalpha expression and secretion in 3T3-L1 cells, Biochimica Et Biophysica Acta: International Journal of Biochemistry and Biophysics, 1803, (10) pp. 1131 - 1141. ISSN 0006-3002 (2010) [Refereed Article]

DOI: doi:10.1016/j.bbamcr.2010.06.012

Abstract

S-resistin is a non-secretable resistin spliced variant described in white adipose tissue from Wistar rats. Since resistin has been implicated in adipogenesis regulation, here we have investigated the possible role of this new isoform in this process. For that, we have studied the adipocyte development in 3T3-L1 pre-adipocyte cell line stably expressing s-resistin and resistin. Both isoforms are able to restrain 3T3-L1 pre-adipocyte differentiation though affecting differently the expression pattern of pro-adipogenic transcription factors such CCAAT/enhancer binding proteins alpha and beta (C/EBPalpha and C/EBPbeta) and peroxisome proliferator-activated receptor gamma (PPARgamma), as well of proteins implicated in lipid metabolism such perilipin, fatty acid synthase (FAS), adipocyte lipid binding protein (ALBP/aP2) and carnitine palmitoyltransferase1 (CPT1). Likewise, both resistin isoforms impair insulin-stimulated glucose transport by decreasing glucose transport 4 (GLUT4) expression but to a different degree. In addition, s-resistin expressing 3T3-L1 cells display other remarkable differences. Thus, in these cells, endogenous resistin expression falls down while tumor necrosis factor alpha (TNFalpha) and interleukine 6 (IL-6) productions are increased along differentiation. These findings indicate that s-resistin isoform also impairs adipocyte differentiation affecting the expression pattern of key pro-adipogenic transcription factors and insulin sensitivity. Additionally, s-resistin may play a role in inflammatory processes.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Metabolism
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Diabetes
Author:Fernandez-Martos, CM (Dr Carmen Fernandez-Martos)
ID Code:86004
Year Published:2010
Web of Science® Times Cited:7
Deposited By:Medicine (Discipline)
Deposited On:2013-08-20
Last Modified:2013-08-20
Downloads:0

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