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Microfluidic primary culture model of the lower motor neuron-neuromuscular junction circuit
Citation
Southam, KA and King, AE and Blizzard, CA and McCormack, GH and Dickson, TC, Microfluidic primary culture model of the lower motor neuron-neuromuscular junction circuit, Journal of Neuroscience Methods, 218, (2) pp. 164-169. ISSN 0165-0270 (2013) [Refereed Article]
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Copyright Statement
Copyright 2013 Elsevier
DOI: doi:10.1016/j.jneumeth.2013.06.002
Abstract
Modelling the complex process of neuromuscular signalling is key to understanding not only normal circuit function but also importantly the mechanisms underpinning a range of degenerative diseases. We describe a novel in vitro model of the lower motor neuron-neuromuscular junction circuit, incorporating primary spinal motor neurons, supporting glia and skeletal muscle. This culture model is designed to spatially mimic the unique anatomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention. This model is suitable for use in conjunction with a range of downstream experimental approaches and could also be modified to utilise other cellular sources including appropriate immortal cell lines, cells derived from transgenic models of disease and also patient derived stem cells. © 2013 Elsevier B.V.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Microfluidic, Motor neuron, Muscle, Neuromuscular junction, Cell culture |
| Research Division: | Medical and Health Sciences |
| Research Group: | Neurosciences |
| Research Field: | Cellular Nervous System |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Nervous System and Disorders |
| Author: | Southam, KA (Dr Katherine Southam) |
| Author: | King, AE (Associate Professor Anna King) |
| Author: | Blizzard, CA (Dr Catherine Blizzard) |
| Author: | McCormack, GH (Mr Graeme McCormack) |
| Author: | Dickson, TC (Professor Tracey Dickson) |
| ID Code: | 85905 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 29 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2013-08-14 |
| Last Modified: | 2018-11-08 |
| Downloads: | 0 |
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