Modelling the complex process of neuromuscular signalling is key to understanding not only normal circuit function but also importantly the mechanisms underpinning a range of degenerative diseases. We describe a novel in vitro model of the lower motor neuron-neuromuscular junction circuit, incorporating primary spinal motor neurons, supporting glia and skeletal muscle. This culture model is designed to spatially mimic the unique anatomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention. This model is suitable for use in conjunction with a range of downstream experimental approaches and could also be modified to utilise other cellular sources including appropriate immortal cell lines, cells derived from transgenic models of disease and also patient derived stem cells. © 2013 Elsevier B.V.

Item Type:	Refereed Article
Keywords:	Microfluidic, Motor neuron, Muscle, Neuromuscular junction, Cell culture
Research Division:	Medical and Health Sciences
Research Group:	Neurosciences
Research Field:	Cellular Nervous System
Objective Division:	Health
Objective Group:	Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:	Nervous System and Disorders
Author:	Southam, KA (Dr Katherine Southam)
Author:	King, AE (Associate Professor Anna King)
Author:	Blizzard, CA (Dr Catherine Blizzard)
Author:	McCormack, GH (Mr Graeme McCormack)
Author:	Dickson, TC (Professor Tracey Dickson)
ID Code:	85905
Year Published:	2013
Web of Science® Times Cited:	29
Deposited By:	Menzies Institute for Medical Research
Deposited On:	2013-08-14
Last Modified:	2017-11-06
Downloads:	0