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The diatom genus Pseudo-nitzschia (Bacillariophyceae) in New South Wales, Australia: Morphotaxonomy, molecular phylogeny, toxicity, and distribution

Citation

Ajani, P and Murray, S and Hallegraeff, GM and Lundholm, N and Gillings, M and Brett, S and Armand, L, The diatom genus Pseudo-nitzschia (Bacillariophyceae) in New South Wales, Australia: Morphotaxonomy, molecular phylogeny, toxicity, and distribution, Journal of Phycology, 49, (4) pp. 765-785. ISSN 0022-3646 (2013) [Refereed Article]


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Copyright Statement

Copyright 2013 Phycological Society of America

DOI: doi:10.1111/jpy.12087

Abstract

Species belonging to the potentially harmful diatom genus Pseudo-nitzschia, isolated from 16 localities (31 sampling events) in the coastal waters of south-eastern Australia, were examined. Clonal isolates were characterized by (i) light and transmission electron microscopy; (ii) phylogenies, based on sequencing of nuclear-encoded ribosomal deoxyribonucleic acid (rDNA) regions and, (iii) domoic acid (DA) production as measured by liquid chromatography-mass spectrometry (LC-MS/MS). Ten taxa were unequivocally confirmed as Pseudo-nitzschia americana, P. arenysensis, P. calliantha, P. cuspidata, P. fraudulenta, P. hasleana, P. micropora, P. multiseries, P. multistriata, and P. pungens. An updated taxonomic key for south-eastern Australian Pseudo-nitzschia is presented. The occurrence of two toxigenic species, P. multistriata (maximum concentration 11 pg DA per cell) and P. cuspidata (25.4 pg DA per cell), was documented for the first time in Australia. The Australian strains of P. multiseries, a consistent producer of DA in strains throughout the world, were nontoxic. Data from 5,888 water samples, collected from 31 oyster-growing estuaries (2,000 km coastline) from 2005 to 2009, revealed 310 regulatory exceedances for "Total Pseudo-nitzschia," resulting in six toxic episodes. Further examination of high-risk estuaries revealed that the "P. seriata group" had highest cell densities in the austral summer, autumn, or spring (species dependent), and lowest cell densities in the austral winter, while the "P. delicatissima group" had highest in winter and spring. Β© 2013 Phycological Society of America.

Item Details

Item Type:Refereed Article
Keywords:algal blooms 2
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:85874
Year Published:2013
Web of Science® Times Cited:6
Deposited By:IMAS Research and Education Centre
Deposited On:2013-08-12
Last Modified:2014-12-18
Downloads:5 View Download Statistics

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