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First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization

Citation

Ajani, P and Murray, S and Hallegraeff, GM and Brett, S and Armand, L, First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization, Phycological Research, 61, (3) pp. 237-248. ISSN 1322-0829 (2013) [Refereed Article]


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Copyright Statement

Copyright 2013 Japanese Society of Phycology

DOI: doi:10.1111/pre.12020

Abstract

Pseudo-nitzschiaH. Peragallo is a marine diatom genus found worldwide in polar, temperate, subtropical and tropical waters. It includes toxigenic representatives that produce domoic acid (DA), a neurotoxin responsible for Amnesic Shellfish Poisoning. In this study we characterized two species of Pseudo-nitzschia collected from Port Stephens and the Hawkesbury River (south eastern Australia) previously unreported from Australian waters. Clonal isolates were sub-sampled for (i) light and transmission electron microscopy; (ii) DNA sequencing, based on the nuclear-encoded partial large subunit ribosomal RNA gene and internal transcribed spacer (ITS)-ITS1, 5.8S and ITS2 rDNA regions and, (iii) DA production as measured by liquid chromatography-mass spectrometry. Morphological and molecular data unambiguously revealed the species to be Pseudo-nitzschia microporaPriisholm, Moestrup & Lundholm (Port Stephens) and Pseudo-nitzschia hasleanaLundholm (Hawkesbury River). This is the first report of the occurrence of these species from the Southern Hemisphere and the first report of P. micropora in warm-temperate waters. Cultures of P. micropora, tested for DA production for the first time, proved to be non-toxic. Similarly, no detectable toxin concentrations were observed for P. hasleana. Species resolution and knowledge on the toxicity of local Pseudo-nitzschia species has important implications for harmful algal bloom monitoring and management. Β© 2013 Japanese Society of Phycology.

Item Details

Item Type:Refereed Article
Keywords:algal blooms
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:85872
Year Published:2013
Web of Science® Times Cited:9
Deposited By:IMAS Research and Education Centre
Deposited On:2013-08-12
Last Modified:2014-06-05
Downloads:0

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