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Ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley
Citation
Wu, H and Shabala, L and Barry, K and Zhou, M and Shabala, S, Ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley, Physiologia Plantarum, 149, (4) pp. 515-527. ISSN 0031-9317 (2013) [Refereed Article]
Copyright Statement
Copyright 2013 Scandinavian Plant Physiology Society
Abstract
This work investigated the importance of the ability of leaf mesophyll
cells to control K+ flux across the plasma membrane as a trait conferring
tissue tolerance mechanism in plants grown under saline conditions. Four
wheat (Triticum aestivum and Triticum turgidum) and four barley (Hordeum
vulgare) genotypes contrasting in their salinity tolerance were grown under
glasshouse conditions. Seven to 10-day-old leaves were excised, and net K+
and H+ fluxes were measured from either epidermal or mesophyll cells upon
acute 100mM treatment (mimicking plant failure to restrict Na+ delivery
to the shoot) using non-invasive microelectrode ion flux estimation (the
MIFE) system. To enable net ion flux measurements from leaf epidermal
cells, removal of epicuticular waxes was trialed with organic solvents. A
series of methodological experiments was conducted to test the efficiency
of different methods of wax removal, and the impact of experimental
procedures on cell viability, in order to optimize the method. A strong
positive correlation was found between plants’ ability to retain K+ in salt-treated leaves and their salinity tolerance, in both wheat and especially barley. The observed effects were related to the ionic but not osmotic component
of salt stress. Pharmacological experiments have suggested that voltage-gated
K+-permeable channels mediate K+ retention in leaf mesophyll upon elevated
NaCl levels in the apoplast. It is concluded that MIFE measurements of NaCl induced K+ fluxes from leaf mesophyll may be used as an efficient screening
tool for breeding in cereals for salinity tissue tolerance.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | mesophyll, salinity tolerance, wheat, barley |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant pathology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Wu, H (Mr Honghong Wu) |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Barry, K (Associate Professor Kara Barry) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 85625 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 76 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2013-07-24 |
| Last Modified: | 2014-04-08 |
| Downloads: | 0 |
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