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Characterization of a type IV bundle-forming pilus (SFP) from a gastroenteritis-associated strain of Aeromonas veronii biovar sobria

Citation

Kirov, SM and Sanderson, K, Characterization of a type IV bundle-forming pilus (SFP) from a gastroenteritis-associated strain of Aeromonas veronii biovar sobria, Microbial Pathogenesis, 21, (1) pp. 23-34. ISSN 0882-4010 (1996) [Refereed Article]

DOI: doi:10.1006/mpat.1996.0039

Abstract

The colonization mechanisms of enteropathogenic Aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. This study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of Aeromonas veronii biovar sobria. SDS-PAGE analysis (various conditions) of pill preparations yielded a pilin protein band of ~21 kDa. Its N-terminal amino acid sequence was unambiguous and homologous with those of type IV pilins. Immunogold electron microscopy with rabbit antisera produced against this pi[in protein (SFP) decorated single pill and rope-like bundles of pill on the bacterial surface. These were seen more frequently on strains grown at 22°C compared with 37°C and in liquid rather than on solid medium. SFP was not detected on any of 104 strains of Aeromonas (different species and sources) from our culture collection, although morphologically similar structures were seen on a number of these strains. This finding and differences among other published amino acid sequences show that Aeromonas type IV pill are antigenically diverse. Bundle-forming type IV 'class B' pill are important in the virulence of other enteropathogenic bacteria. The N-terminal amino acid sequence of the Aeromonas SFP, however, showed closer homology to the type IV 'class A' pilins. Studies are in progress to investigate the role of SFP in Aeromonas virulence.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Kirov, SM (Associate Professor Sylvia Kirov)
Author:Sanderson, K (Dr Kevin Sanderson)
ID Code:8559
Year Published:1996
Web of Science® Times Cited:20
Deposited By:Pathology
Deposited On:1996-08-01
Last Modified:2011-08-19
Downloads:0

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