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Genetic variance of malate transporter gene in barley (Hordeum vulgare L.)


Bian, M and Broughton, S and Walters, I and Zhou, M and Sun, D and Li, C, Genetic variance of malate transporter gene in barley (Hordeum vulgare L.), The 16th Australian Barley Technical Symposium, 8-11 September 2013, Melbourne, Victoria (2013) [Non Refereed Conference Paper]


HvALMT1 encodes a malate transporter responsible for malic acid efflux in barley on 2H. The gene was characterized to contribute to anion homeostasis and stomatal adjustment. However, it has been debated for some time whether the gene function is related to Al tolerance. We developed thirty-six pairs of primers covering whole gene sequence and tested polymorphisms in 335 accessions with different Al tolerance. Phenotypic variation was evaluated in native acid soil and limed acid soil. Ten pairs of primers (4 in the upstream region, 6 in the coding region,) showed polymorphisms. Stepwise regression analysis revealed one gene-specific marker, UA21, was significantly correlated with phenotypic variation of root length in acid soil (r = 0.301, p < 0.01) and relative root length (root length in acid soil compared with that in acid soil treated with lime) (r = 0.246, p < 0.01). The results demonstrated that malate transporter gene may play a role for acids soil tolerance in some of the germplasm.

Item Details

Item Type:Non Refereed Conference Paper
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Bian, M (Ms Miao Bian)
UTAS Author:Broughton, S (Dr Sonya Broughton)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:85568
Year Published:2013
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-07-18
Last Modified:2014-04-29

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